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<p>Steve,</p>
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<p> It is persistent, it changes with time (short term time scale - between record images a fast as you can record them) and we've tried wiggling cables, re-seating connectors, etc. I will take a look tomorrow and see what happens if we wiggle, bump, push
on things during live mode...<br>
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-- <br>
<div name="divtagdefaultwrapper" style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:; margin:0">
David Gene Morgan<br>
Electron Microscopy Center<br>
047D Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
http://iubemcenter.indiana.edu</div>
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<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" color="#000000" face="Calibri, sans-serif"><b>From:</b> Ludtke, Steven J <sludtke@bcm.edu><br>
<b>Sent:</b> Thursday, February 4, 2016 7:55 PM<br>
<b>To:</b> Jaap Brink<br>
<b>Cc:</b> Morgan, David Gene; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] more on the odd CCD image</font>
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<div>I agree it was subtler in our case, but if I recall when we decided it might be the cable and wiggled it, I think we did get more visible noise, and this is how we decided it was actually the problem(?) (This was maaany years ago, David) Anyway, if
it may be the cable, then wiggling it while in a faster live mode may reveal something. I'm sure Gatan will be able to say more if the problem is persistent not transitory. I think I must have missed the original message, so I don't know what you said originally.
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<div class="">----------------------------------------------------------------------------<br class="">
Steven Ludtke, Ph.D.<br class="">
Professor, Dept. of Biochemistry and Mol. Biol. Those who do</div>
<div class="">Co-Director National Center For Macromolecular Imaging<span class="Apple-tab-span" style="white-space:pre">
</span> ARE</div>
<div class="">Baylor College of Medicine The converse<br class="">
<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a> -or- <a href="mailto:stevel@alumni.caltech.edu" class="">stevel@alumni.caltech.edu</a> also applies<br class="">
<a href="http://ncmi.bcm.edu/~stevel" class="">http://ncmi.bcm.edu/~stevel</a><br class="">
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<div class="">On Feb 4, 2016, at 6:40 PM, Jaap Brink <<a href="mailto:jbrink@jeol.com" class="">jbrink@jeol.com</a>> wrote:</div>
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<div class="">Steve, if I recall that event correctly weren't there a lot of spikes in the FFT? I don't remember whether or not the real space image looked this obviously bad. </div>
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Jaap
<div class="">----
<div class="">Jacob Brink <span class="" style="font-size:13pt">Ph.D.</span></div>
<div class=""><span class="" style="font-size:13pt">TEM Product Manager Life Sciences</span></div>
<div class=""><span class="" style="font-size:13pt">JEOL USA, Inc., Peabody MA 01960</span></div>
<div class=""><span class="" style="font-size:13pt">(978) 317-9561</span></div>
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</span></div>
<div class="">Sent from mobile device. Please excuse typos and this reply's brevity as I'm using all thumbs to type :-)</div>
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On Feb 4, 2016, at 7:27 PM, Ludtke, Steven J <<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a>> wrote:<br class="">
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<div class=""><span class="">Electrical noise due to a loose/bad cable? I seem to recall a case where we had a bad cable connection due to being periodically "bumped" or somesuch. This is a bit more extreme than I recall seeing, but certainly if there is a
grounding issue or a bad cable all sorts of electrical badness can occur. If you know the readout rate of the detector you can estimate the frequency of the noise you're seeing.</span><br class="">
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<span class="">Of course it could be something else :^)</span><br class="">
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<span class="">----------------------------------------------------------------------------</span><br class="">
<span class="">Steven Ludtke, Ph.D.</span><br class="">
<span class="">Professor, Dept. of Biochemistry and Mol. Biol. Those who do</span><br class="">
<span class="">Co-Director National Center For Macromolecular Imaging ARE</span><br class="">
<span class="">Baylor College of Medicine The converse</span><br class="">
<span class=""><a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a> -or- <a href="mailto:stevel@alumni.caltech.edu" class="">stevel@alumni.caltech.edu</a> also applies</span><br class="">
<span class=""><a href="http://ncmi.bcm.edu/~stevel" class="">http://ncmi.bcm.edu/~stevel</a></span><br class="">
<span class=""></span><br class="">
<blockquote type="cite" class=""><span class="">On Feb 4, 2016, at 6:08 PM, David Gene Morgan <<a href="mailto:dagmorga@indiana.edu" class="">dagmorga@indiana.edu</a>> wrote:</span><br class="">
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<blockquote type="cite" class=""><span class="">Hi,</span><br class="">
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<blockquote type="cite" class=""><span class=""> Someone pointed out that the image I attached to my original post was not passed by the mail server. You can see that image at:</span><br class="">
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<blockquote type="cite" class=""><span class=""> <a href="http://iubemcenter.indiana.edu/oddImageFromUltrascan890.html" class="">http://iubemcenter.indiana.edu/oddImageFromUltrascan890.html</a></span><br class="">
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<blockquote type="cite" class=""><span class="">-- </span><br class="">
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<blockquote type="cite" class=""><span class=""> David Gene Morgan</span><br class="">
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<blockquote type="cite" class=""><span class=""> Electron Microscopy Center</span><br class="">
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<blockquote type="cite" class=""><span class=""> 047D Simon Hall</span><br class="">
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<blockquote type="cite" class=""><span class=""> IU Bloomington</span><br class="">
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<blockquote type="cite" class=""><span class=""> 812 856 1457 (office)</span><br class="">
</blockquote>
<blockquote type="cite" class=""><span class=""> 812 856 3221 (3200)</span><br class="">
</blockquote>
<blockquote type="cite" class=""><span class=""> <a href="http://iubemcenter.indiana.edu/" class="">http://iubemcenter.indiana.edu</a></span><br class="">
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