<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">As associate editor of Scientific Reports, I, for once, entirely agree with you, Ed! ;)<div class="">Indeed, in French we have a rather dirty saying: “Faudrait voir à ne pas péter plus haut que son cul!”</div><div class=""><span style="background-color: rgb(255, 255, 255);" class="">Just my own two cents (which are indeed worth less & less and may well be worth nothing more from me at all, after you all have read this!)</span></div><div class=""><span style="background-color: rgb(255, 255, 255);" class="">Bonne fin de Dimanche!</span></div><div class=""><span style="background-color: rgb(255, 255, 255);" class="">Best,</span></div><div class=""><span style="background-color: rgb(255, 255, 255);" class="">Ariel.<br class=""></span><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Ariel J. Blocker, PhD, FRSB</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Reader in Microbiology </span></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Wellcome Trust Investigator</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Schools of Cellular & Molecular Medicine and Biochemistry</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Biomedical Sciences Building</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">University of Bristol</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">University Walk</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Bristol BS8 1TD</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">United Kingdom</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Email: <a href="mailto:ariel.blocker@bristol.ac.uk" class="">ariel.blocker@bristol.ac.uk</a></span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Tel office (D39a): +44 117 33 12063</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Tel wetlab (D57/D59): +44 117 33 12404</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Tel wetlab office (D53): +44 117 33 12059</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Tel drylab (D51): +44 117 33 12097</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Tel Tecnai20: +44 117 33 12357</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Dept. fax: +44 117 3312091</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Room: D39a</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Personal webpage: </span><a href="http://www.bristol.ac.uk/cellmolmed/research/infect-immune/blocker.html" class="">http://www.bristol.ac.uk/cellmolmed/research/infect-immune/blocker.html</a><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Group page: </span><a href="http://www.bristol.ac.uk/cellmolmed/research/infect-immune/blocker/" class="">http://www.bristol.ac.uk/cellmolmed/research/infect-immune/blocker/</a><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Postgraduate students searching for a PhD please look here:</span><br style="font-size: 12px; orphans: 2; widows: 2;" class=""><a href="http://www.bris.ac.uk/fmvs/gradschool/" class="">http://www.bris.ac.uk/fmvs/gradschool/</a></div><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><span style="font-size: 12px; orphans: 2; widows: 2;" class="">Twitter: @T3Secreton</span></div></div></div></div></div></div></div></div></div></div></div></div>
</div>
<br class=""><div><blockquote type="cite" class=""><div class="">On 31 Jan 2016, at 16:34, Edward Egelman <<a href="mailto:egelman@virginia.edu" class="">egelman@virginia.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class=""> Why are people using "Nature, Scientific Reports"? The journal's name is Scientific Reports, and Nature is the publisher. By this logic I could say that I was the Editor-in-Chief of Cell, Biophysical Journal, since Cell is the publisher of BJ. Not to rain on Marin's parade, but If one looks at Peer Review Policy for Nature publications, it states:</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class="">"The policy outlined on this page applies to Nature journals (those with the word "Nature" in their title). NPG publishes many other journals, each of which has separate publication policies described on its website."</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class="">The policy for Nature journals goes on to explain the need for novelty, significance and likely impact. In stark contrast, this is what they say about Scientific Reports:</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class="">"To be considered for publication in Scientific Reports, a paper must be technically sound original research, without any requirement for impact or a conceptual advance."</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class=""> Just my two cents (which are worth less and less).</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class="">Ed</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><div class="moz-cite-prefix" style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">On 1/30/16 8:07 AM, Marin van Heel wrote:<br class=""></div><blockquote cite="mid:56ACB5AE.5010807@googlemail.com" type="cite" style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><div class="moz-cite-prefix"><br class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">Dear Nancy,<o:p class=""></o:p></span></div><span style="font-size: 10pt;" class=""><o:p class=""></o:p></span><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">What Sjors is suggesting is a simplified version of what we suggested in:<o:p class=""></o:p></span></div><span style="font-size: 10pt;" class=""><o:p class=""></o:p></span><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">"A posteriori correction of camera characteristics from large image data sets", P. Afanasyev et al, Nature, Scientific Reports.<o:p class=""></o:p></span><span style="font-size: 10pt;" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">The camera correction should preferably include the subtraction of the average background and the normalization of the standard deviations of the pixel vectors, especially if you are operating your DE camera in integrating mode. The movie alignments can improve significantly after the a posteriori camera correction.<span class=""> <span class="Apple-converted-space"> </span></span>The other aspect is the FRC validation of the camera correction. The FRC is then not used as a ‘gold standard’ 2D resolution criterion but rather as an indicator of the independence of different images collected with the same sensor. The FRC should not cross the 3-sigma threshold indicating the expected random noise fluctuations around FRC=0. For details see the paper.<o:p class=""></o:p></span></div><span style="font-size: 10pt;" class=""><o:p class=""></o:p></span><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">If you want to test the a posteriori camera correction programs, contact<span class="Apple-converted-space"> </span><a moz-do-not-send="true" class="moz-txt-link-abbreviated" href="mailto:michael@imagescience.de" style="color: purple; text-decoration: underline;"></a><a class="moz-txt-link-abbreviated" href="mailto:michael@imagescience.de" style="color: purple; text-decoration: underline;">michael@imagescience.de</a>!<o:p class=""></o:p></span></div><span style="font-size: 10pt;" class=""><o:p class=""></o:p></span><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">Hope this helps!<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class="">Marin<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria; line-height: normal;" class=""><span style="font-size: 10pt;" class=""><o:p class=""> </o:p></span></div>=====================================<br class=""><br class="">On 28/01/2016 00:48, Nancy Meyer wrote:<br class=""></div><blockquote cite="mid:7B6DD4F49731AB4691DC21234C3BAE985641108A@EXMB06.ohsu.edu" type="cite" class=""><pre wrap="" class="">Again, thank you for the reassuring response! Maybe I've lucked out with similar image intensities in previous datasets - I should probably look at a few min/max, mean, and stdev values to convince myself.
I was pretty careful to remove particles on that horiz artefact and if restoring their inclusion is the biggest gain from redoing gain correction, I can maybe let that slide for now. I did just see "A posteriori correction of camera
characteristics from large image data sets", P. Afanasyev et al, Nature, Scientific Reports, after looking up your recommendation - looks like they've implemented it in Imagic - wonder if you could do the same correction in just ImageJ...
Thank you for taking the time to look at the images... slowly getting the hang of Relion, but the input is so invaluable!
Best,
Nancy
______________________________________
From: Sjors Scheres [<a moz-do-not-send="true" class="moz-txt-link-abbreviated" href="mailto:scheres@mrc-lmb.cam.ac.uk" style="color: purple; text-decoration: underline;">scheres@mrc-lmb.cam.ac.uk</a>]
Sent: Wednesday, January 27, 2016 1:10 PM
To: Nancy Meyer
Cc: <a moz-do-not-send="true" class="moz-txt-link-abbreviated" href="mailto:ccpem@jiscmail.ac.uk" style="color: purple; text-decoration: underline;">ccpem@jiscmail.ac.uk</a>
Subject: Re: [ccpem] Adequate particle normalization prior to 2D classification?
Hi Nancy,
The particles look perfectly fine to me. The overall differences in
greyscale you see is because each of the particles is scaled from white to
black with their own min and max values, which are susceptible to
outliers. The mean will probably be perfectly fine. You can get rid of
this with removing white and black dust, but it will not be necessary to
do so.
The classes also look quite OK. Just select all the nice ones and proceed
with another 2D classification or go into 3D already. Some details are
already coming up, so the 3D structure will look nice.
The micrographs show a sign of a bad gain correction. If you have many of
them (> several hundreds) you could correct the gain a-posteriori by
calculating the average of all micrographs and then divide each micrograph
by that average. This should get rid of the horizontal artefact (as long
as it is present in all micrographs). That may save some particles that
lie on that line.
HTH,
Sjors
</pre><br class=""></blockquote><br class=""><pre class="moz-signature" cols="72">--
================================================================
Prof Dr Ir Marin van Heel
Professor of Cryo-EM Data Processing
Leiden University
NeCEN Building Room 05.27
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2333 CC Leiden
The Netherlands
Tel. NL: +31(0)715271424 // Mobile NL: +31(0)652736618
Skype: Marin.van.Heel
email: marin.vanheel(A_T)<a href="http://gmail.com" class="">gmail.com</a>
and: mvh.office(A_T)<a href="http://gmail.com" class="">gmail.com</a>
</pre><br class=""><fieldset class="mimeAttachmentHeader"></fieldset><br class=""><pre wrap="" class="">_______________________________________________
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</pre></blockquote><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><div class="moz-signature" style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">--<span class="Apple-converted-space"> </span><br class=""><div class="WordSection1" style="page: WordSection1;"><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><span class=""><o:p class=""> </o:p></span></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><span class=""><br class=""><br class=""></span><span style="font-size: 10pt; font-family: Times;" class=""><o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class="">Edward H. Egelman, Ph.D.<u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class="">Harrison Distinguished Professor<u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class="">Dept. of Biochemistry and Molecular Genetics<u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class="">University of Virginia<u5:p class=""></u5:p><o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><u5:p class=""> </u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class="">President<u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class="">Biophysical Society<u5:p class=""></u5:p><o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><u5:p class=""> </u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><span class="GramE">phone</span>: 434-924-8210<u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><span class="GramE">fax</span>: 434-924-5069<u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><a class="moz-txt-link-abbreviated" href="mailto:egelman@virginia.edu" style="color: purple; text-decoration: underline;">egelman@virginia.edu</a><u5:p class=""></u5:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><a href="http://www.people.virginia.edu/%7Eehe2n" style="color: purple; text-decoration: underline;" class=""></a><a class="moz-txt-link-freetext" href="http://www.people.virginia.edu/~ehe2n" style="color: purple; text-decoration: underline;">http://www.people.virginia.edu/~ehe2n</a></div><u5:p class=""></u5:p><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: Cambria;" class=""><span style="font-size: 10pt; font-family: Times;" class=""><br class=""><o:p class=""></o:p></span></div></div></div></div><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><span style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); float: none; display: inline !important;" class="">3dem mailing list</span><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br style="font-family: Helvetica; font-size: 14px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);" class=""></div></blockquote></div><br class=""></div></body></html>