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<p class="MsoNormal">Dear all,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Has anyone performed electron cryo-tomography on polymers before? I’m curious about what dose those sorts of samples can withstand, and whether a different tilt scheme is beneficial. There’s some info in the paper below but it’s simulated
data, so it would be great to hear from anyone with practical experience.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">On Resolution in Electron Tomography of Beam Sensitive Materials. D. Chen, H. Friedrich and G. de With. J. Phys. Chem. C, 2014, 118 (2), pp 1248–1257. DOI: 10.1021/jp409185u<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks in advance,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-GB">Thom</span><b><u><span style="font-size:10.0pt;color:gray;mso-fareast-language:EN-GB"><o:p></o:p></span></u></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#365F91;mso-fareast-language:EN-GB">Dr. Thomas H. Sharp<o:p></o:p></span></b></p>
<p class="MsoNormal"><span style="font-size:8.0pt;color:gray;mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB">Leiden University Medical Center<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB">Section Electron Microscopy<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="NL" style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB">Leiden, NL<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="NL" style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:Wingdings;color:gray;mso-fareast-language:EN-GB">(</span><span lang="NL" style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB"> 0031-(0)-7152-69499
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:Wingdings;color:gray;mso-fareast-language:EN-GB">*</span><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB">
</span><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB"><a href="mailto:t.sharp@lumc.nl"><span lang="NL" style="color:gray;text-decoration:none">t.sharp@lumc.nl</span></a></span><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB">
<span lang="NL"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial","sans-serif";color:gray;mso-fareast-language:EN-GB"><a href="https://electronmicroscopy.lumc.nl/"><span style="color:gray">https://electronmicroscopy.lumc.nl/</span></a></span><span style="color:gray;mso-fareast-language:EN-GB"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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