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--></style></head><body lang=EN-US><div class=WordSection1><p class=MsoNormal>Dear Zhongjun,</p><p class=MsoNormal>You can try this to fix your problem: simply copy your .mrc file into .ccp4 file, open the .ccp4 map and your pdb in Chimera, re-fit the pdb into the map, and save the pdb relative to the map. Then the .ccp4 map and the pdb should be well aligned in Coot. </p><p class=MsoNormal>Good luck!</p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Best regards, <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Xinghong Dai<br>Postdoctoral Scholar<br>Department of Molecular and Medical Pharmacology, UCLA<br>+1 310 447 4330</p><p><o:p> </o:p></p><p><o:p> </o:p></p><div style='mso-element:para-border-div;border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;border:none;padding:0in'><br><b>From: </b>Zhongjun Hu<br><b>Sent: </b>Saturday, November 28, 2015 5:21 PM<br><b>To: </b>3dem@ncmir.ucsd.edu<br><b>Subject: </b>[3dem] map and pdb are off aligned in coot.</p></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><span style='font-size:12.0pt;font-family:"Times New Roman",serif'><o:p> </o:p></span></p><div><div><div><div><p class=MsoNormal style='margin-bottom:12.0pt'><span style='font-size:12.0pt;font-family:"Times New Roman",serif'>Hi, everyone:</span><span style='font-size:12.0pt;font-family:"Times New Roman",serif'><o:p></o:p></span></p></div><p class=MsoNormal style='margin-bottom:12.0pt'><span style='font-size:12.0pt;font-family:"Times New Roman",serif'>I meet a very strange problem that my pdb file and mrc file are well aligned in Chimera, but off aligned in Coot. This is very strange to me, because two month ago, it did not have this problem. Now I cannot fix it. Could you please give me some idea? Thanks.<o:p></o:p></span></p></div><p class=MsoNormal style='margin-bottom:12.0pt'><span style='font-size:12.0pt;font-family:"Times New Roman",serif'>Best,<o:p></o:p></span></p></div></div><p class=MsoNormal><span style='font-size:12.0pt;font-family:"Times New Roman",serif'>Zhongjun<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>