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<div style="font-family:Tahoma; font-size:13px">Valorie Bowman<br>
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vdb@purdue.edu<br>
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Senior Research Electron Microscopist<br>
Purdue Cryo EM Facility<br>
Purdue University</div>
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<div style="direction: ltr;" id="divRpF700769"><font face="Tahoma" color="#000000" size="2"><b>From:</b> Bowman, Valorie D<br>
<b>Sent:</b> Friday, October 23, 2015 8:17 AM<br>
<b>To:</b> Michael Saur<br>
<b>Subject:</b> RE: [3dem] 3-D printable CARTRIDGE boxes!<br>
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<div style="direction:ltr; font-family:Tahoma; color:#000000; font-size:10pt">Dear Michael,<br>
Fantastic idea! <br>
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And for those of us using a Titan or other microscope with an autoloader requiring grids in cartridges we could also print modified cryo grid boxes with
<i>slots specially sized for cartridges</i>. Thanks to a very talented machinist we have hundreds of cryo grid boxes, but we are constantly running out of those boxes with the wider slots required for grids loaded into cartridges. I've modified several myself
with a drill press, but it's time consuming. <br>
<br>
Imagine being able to print them. Wow, I love it!<br>
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<div style="font-family:Tahoma; font-size:13px">Valorie Bowman<br>
<br>
vdb@purdue.edu<br>
<br>
Senior Research Electron Microscopist<br>
Purdue Cryo EM Facility<br>
Purdue University</div>
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<div id="divRpF146166" style="direction:ltr"><font face="Tahoma" color="#000000" size="2"><b>From:</b> 3dem [3dem-bounces@ncmir.ucsd.edu] on behalf of Michael Saur [2michael.saur@gmail.com]<br>
<b>Sent:</b> Thursday, October 22, 2015 5:59 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] 3-D printable grid boxes<br>
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<div>Dear community,<br class="">
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<div class="">if anybody is interested, I would like to share 3-D printable grid boxes with you.</div>
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<div class="">We recently purchased an FDM 3-D printer and with it came new possibilities of supplying our cryo-EM facility with useful things. Since grid boxes are fairly expensive for what they are, I modelled some and printed them. After tweaking the design
in terms of screw hole size etc., I now have a working print which we have successfully used in our lab for a couple of weeks. The grid boxes survive cooling and even heating up with a hair dryer many times without any problems. As you can see in the attached
images, I modelled the standard diamond shape but also a variant of the available cross-shaped holes (which for us are much easier to use since grids are never sticking to the side so that you damage them with the tweezers). Printing is fairly easy and takes
10 min per grid box. We used standard PLA filament so far. </div>
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<div class="">The material costs are approx. 0.02 Euro for the box, 0.2 Euro for the screw and whatever you spend for lids. Plus electricity and running costs for the printer of course (which should be negligible).</div>
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<div class="">I can provide you with the actual .blend files of the design (in case your printer needs some tweaking of the central hole for fitting the screws), the CURA print profile that worked best for me and/or the stl-file. Just write me an E-mail (directly
to me so that we don’t spam the group) telling me which files or further information you would need and I’ll send it to you. </div>
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<div class="">Of course, if there is any legal issue with me providing these things, please also let me know ASAP! I had a look in patent data bases, but I could not find anything. Since, to my knowledge, these boxes are around in the same design since the
‘70s, I don’t expect any patent issues. Still, please let me know if there is anything and I will stop any sharing of the designs.</div>
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<div class="">Cheers,</div>
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<div class="">Michael</div>
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Michael Saur, Dr. rer. nat. <br class="">
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<div class=""> Max Planck Institute for Molecular Physiology </div>
<div class=""> Department III, Structural Biochemistry </div>
<div class=""> Raunser lab <br class="">
Otto-Hahn-Straße 11</div>
<div class=""> Room B2.25 <br class="">
44227 Dortmund<br class="">
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Phone: +49 231 - 133 2312 <br class="">
E-mail: <a href="mailto:michael.saur@mpi-dortmund.mpg" class="" target="_blank">michael.saur@mpi-dortmund.mpg</a>.de <br class="">
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