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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Dear Michael,<br>
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I don't see a problem. These are NOT based on their (FEI) cartridge boxes, so there should be no copyright issue. These are cut from a regular large grid box with a hole saw, notched with a drill press, and then the diamond shape holes modified with the drill
press and threads added for the lids. The little bit of the diamond left below it keeps the cartridge sitting upright, and the wider holes allow you access with the special loading forceps.<br>
<br>
I've attached a photo.<br>
<br>
Cheers,<br>
<br>
Valorie<br>
<div><br>
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<div style="font-family:Tahoma; font-size:13px">Valorie Bowman<br>
<br>
vdb@purdue.edu<br>
<br>
Senior Research Electron Microscopist<br>
Purdue Cryo EM Facility<br>
Purdue University</div>
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<div style="direction: ltr;" id="divRpF699178"><font face="Tahoma" color="#000000" size="2"><b>From:</b> Michael Saur [michael.saur@mpi-dortmund.mpg.de]<br>
<b>Sent:</b> Friday, October 23, 2015 9:24 AM<br>
<b>To:</b> Bowman, Valorie D<br>
<b>Subject:</b> Re: [3dem] 3-D printable CARTRIDGE boxes!<br>
</font><br>
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<div class="">Hi Valorie,</div>
<div class=""><br class="">
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<div class="">thanks! It would be awesome to have those for the Krios and share them. I mean, I guess making them would not be a problem, but I would be a bit scared of FEI when sharing them. Already with these old boxes I had a long thought about it until
deciding to go for sharing since I couldn’t find any patent.</div>
<div class=""><br class="">
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<div class="">But I also love the possibilities that arise for our facilities with 3-D printers. Sometimes I am feeling like a child in a toy store.</div>
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<div class="">Kind regards</div>
<div class=""><br class="">
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<div class="">Michael</div>
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<div class="">------------------------------------------------------------<br class="">
<br class="">
Michael Saur, Dr. rer. nat. <br class="">
</div>
<div class=""> Max Planck Institute for Molecular Physiology </div>
<div class=""> Department III, Structural Biochemistry <br class="">
Otto-Hahn-Straße 11</div>
<div class=""> Room B2.25 <br class="">
44227 Dortmund<br class="">
<br class="">
Phone: +49 231 - 133 2312 <br class="">
E-mail: <a href="mailto:michael.saur@mpi-dortmund.mpg" class="" target="_blank">michael.saur@mpi-dortmund.mpg</a>.de <br class="">
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<blockquote type="cite" class="">
<div class="">On 23 Oct 2015, at 14:17, Bowman, Valorie D <<a href="mailto:vdb@purdue.edu" class="" target="_blank">vdb@purdue.edu</a>> wrote:</div>
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Dear Michael,<br class="">
Fantastic idea! <span class="Apple-converted-space"> </span><br class="">
<br class="">
And for those of us using a Titan or other microscope with an autoloader requiring grids in cartridges we could also print modified cryo grid boxes with<span class="Apple-converted-space"> </span><i class="">slots specially sized for cartridges</i>. Thanks
to a very talented machinist we have hundreds of cryo grid boxes, but we are constantly running out of those boxes with the wider slots required for grids loaded into cartridges. I've modified several myself with a drill press, but it's time consuming. <span class="Apple-converted-space"> </span><br class="">
<br class="">
Imagine being able to print them. Wow, I love it!<br class="">
<div class=""><br class="">
<div class="" style="font-family:Tahoma; font-size:13px">
<div class="" style="font-family:Tahoma; font-size:13px">Valorie Bowman<br class="">
<br class="">
<a href="mailto:vdb@purdue.edu" class="" target="_blank">vdb@purdue.edu</a><br class="">
<br class="">
Senior Research Electron Microscopist<br class="">
Purdue Cryo EM Facility<br class="">
Purdue University</div>
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<div id="divRpF146166" class="" style="direction:ltr"><font class="" face="Tahoma" size="2"><b class="">From:</b><span class="Apple-converted-space"> </span>3dem [<a href="mailto:3dem-bounces@ncmir.ucsd.edu" class="" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>]
on behalf of Michael Saur [<a href="mailto:2michael.saur@gmail.com" class="" target="_blank">2michael.saur@gmail.com</a>]<br class="">
<b class="">Sent:</b><span class="Apple-converted-space"> </span>Thursday, October 22, 2015 5:59 PM<br class="">
<b class="">To:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" class="" target="_blank">3dem@ncmir.ucsd.edu</a><br class="">
<b class="">Subject:</b><span class="Apple-converted-space"> </span>[3dem] 3-D printable grid boxes<br class="">
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<div class="">Dear community,<br class="">
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<div class="">if anybody is interested, I would like to share 3-D printable grid boxes with you.</div>
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<div class="">We recently purchased an FDM 3-D printer and with it came new possibilities of supplying our cryo-EM facility with useful things. Since grid boxes are fairly expensive for what they are, I modelled some and printed them. After tweaking the design
in terms of screw hole size etc., I now have a working print which we have successfully used in our lab for a couple of weeks. The grid boxes survive cooling and even heating up with a hair dryer many times without any problems. As you can see in the attached
images, I modelled the standard diamond shape but also a variant of the available cross-shaped holes (which for us are much easier to use since grids are never sticking to the side so that you damage them with the tweezers). Printing is fairly easy and takes
10 min per grid box. We used standard PLA filament so far. </div>
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<div class="">The material costs are approx. 0.02 Euro for the box, 0.2 Euro for the screw and whatever you spend for lids. Plus electricity and running costs for the printer of course (which should be negligible).</div>
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<div class="">I can provide you with the actual .blend files of the design (in case your printer needs some tweaking of the central hole for fitting the screws), the CURA print profile that worked best for me and/or the stl-file. Just write me an E-mail (directly
to me so that we don’t spam the group) telling me which files or further information you would need and I’ll send it to you. </div>
<div class=""><br class="">
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<div class="">Of course, if there is any legal issue with me providing these things, please also let me know ASAP! I had a look in patent data bases, but I could not find anything. Since, to my knowledge, these boxes are around in the same design since the
‘70s, I don’t expect any patent issues. Still, please let me know if there is anything and I will stop any sharing of the designs.</div>
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<div class="">Cheers,</div>
<div class=""><br class="">
</div>
<div class="">Michael</div>
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<div class="">------------------------------------------------------------<br class="">
<br class="">
Michael Saur, Dr. rer. nat. <br class="">
</div>
<div class=""> Max Planck Institute for Molecular Physiology </div>
<div class=""> Department III, Structural Biochemistry </div>
<div class=""> Raunser lab <br class="">
Otto-Hahn-Straße 11</div>
<div class=""> Room B2.25 <br class="">
44227 Dortmund<br class="">
<br class="">
Phone: +49 231 - 133 2312 <br class="">
E-mail: <a href="mailto:michael.saur@mpi-dortmund.mpg" class="" target="_blank">michael.saur@mpi-dortmund.mpg</a>.de <br class="">
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