<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;">
<div>Hi Gongpu,</div>
<div><br>
</div>
<div>One more test suggestion: does this behaviour happen with more than one type of specimen carrier (e.g. Membrane carriers vs copper tubes)? I have experienced with two EMPACT2 machines that after a few years of usage the tubes burst if you shoot at 2000
bars and you get a complete pressure failure, but this is not the case here. The solution to this tube problem is simply to reduce a bit the pressure, to around 1900 bars. Since we do CEMOVIS, we can check by electron diffraction that the sample is indeed
vitrified so we are not worried to work slightly below the optimal pressure value.</div>
<div><br>
</div>
<div>Best wishes</div>
<div>Ben</div>
<div><br>
</div>
<span id="OLK_SRC_BODY_SECTION">
<div style="font-family:Calibri; font-size:11pt; text-align:left; color:black; BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 0in; PADDING-RIGHT: 0in; BORDER-TOP: #b5c4df 1pt solid; BORDER-RIGHT: medium none; PADDING-TOP: 3pt">
<span style="font-weight:bold">De : </span>3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Paul Verkade <<a href="mailto:bixpv@bristol.ac.uk">bixpv@bristol.ac.uk</a>><br>
<span style="font-weight:bold">Date : </span>mercredi, 7 octobre 2015 18:00<br>
<span style="font-weight:bold">À : </span>"Zhao, Gongpu" <<a href="mailto:goz1@pitt.edu">goz1@pitt.edu</a>><br>
<span style="font-weight:bold">Cc : </span>"<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>
<span style="font-weight:bold">Objet : </span>Re: [3dem] pressure failure with Leica EM Pact2?<br>
</div>
<div><br>
</div>
<blockquote id="MAC_OUTLOOK_ATTRIBUTION_BLOCKQUOTE" style="BORDER-LEFT: #b5c4df 5 solid; PADDING:0 0 0 5; MARGIN:0 0 0 5;">
<div>
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
Hi Gongpu,
<div class=""><br class="">
</div>
<div class="">Indeed pressure should go up to around 2050 bar.</div>
<div class=""><br class="">
</div>
<div class="">A few things to check (which you probably already did).</div>
<div class="">If you turn the blue knob on the side you can adjust the pressure, try adjusting that.</div>
<div class="">Is the incoming pressure from the compressor OK (about 10bar)?</div>
<div class=""><br class="">
</div>
<div class="">Let me know if it works (or not), otherwise I can think of a few other things to try / check.</div>
<div class=""><br class="">
</div>
<div class="">Good luck,</div>
<div class="">Paul</div>
<div class="">
<div apple-content-edited="true" class=""><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; orphans: 2; text-align: -webkit-auto; widows: 2; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; text-align: -webkit-auto; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; text-align: -webkit-auto; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
<div class="">__________________________________</div>
<div class="">Paul Verkade PhD FRMS</div>
<div class="">Reader in Cell Imaging</div>
<div class="">Wolfson Bioimaging Facility</div>
<div class="">Schools of Biochemistry and Physiology & Pharmacology</div>
<div class="">Biomedical Sciences Building</div>
<div class="">University of Bristol</div>
<div class="">University Walk</div>
<div class="">BS8 1TD Bristol</div>
<div class="">United Kingdom</div>
<div class=""><br class="khtml-block-placeholder">
</div>
<div class="">tel.+44-117-3312179</div>
<div class="">Fax. +44-117-3312168</div>
<div class="">mail: <a href="mailto:p.verkade@bristol.ac.uk" class="">p.verkade@bristol.ac.uk</a></div>
<div class="">websites:</div>
<div class=""><a href="http://www.bristol.ac.uk/biochemistry/research/pv.html" class="">http://www.bristol.ac.uk/biochemistry/research/pv.html</a></div>
<div class=""><a href="http://www.bris.ac.uk/phys-pharm/research/staffresearch/paulverkade.html" class="">http://www.bris.ac.uk/phys-pharm/research/staffresearch/paulverkade.html</a></div>
<div class=""><a href="http://www.bristol.ac.uk/biochemistry/wbif" class="">http://www.bristol.ac.uk/biochemistry/wbif</a></div>
<div class=""><br class="">
</div>
</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></div>
</span></span></span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</span></div>
</div>
</span></div>
</span></div>
</span></div>
</span></div>
</div>
<div>
<blockquote type="cite" class="">
<div class="">On 6 Oct 2015, at 20:42, Zhao, Gongpu <<a href="mailto:goz1@pitt.edu" class="">goz1@pitt.edu</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
Dear All,<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
We are experiencing a pressure failure problem during high pressure freezing using Leica EM Pact . For successful freezing, the curve is smooth and pressure should go up to 2000 bar. But right now, the curve is fuzzy, at some points it drops and the small display
shows the pressure is failure. I have attached an image of the bad curve. Any insights about what might cause the problem and how to fix it would be greatly appreciated? Thank you very much.<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
gongpu<o:p class=""></o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<b class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">GONGPU ZHAO, PH.D.<o:p class=""></o:p></span></b></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">Research Associate<o:p class=""></o:p></span></i></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">Department of Structural Biology<o:p class=""></o:p></span></i></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">University of Pittsburgh School of Medicine<o:p class=""></o:p></span></i></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">3501 5th Ave. RM2054, Pittsburgh, PA 15260<o:p class=""></o:p></span></i></div>
<div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
</div>
<span id="cid:0EA97C78-4396-4082-9A86-DAAA2062C862@users.bris.ac.uk"><pressure_failure.jpg></span><span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">3dem
mailing list</span><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></div>
</blockquote>
</div>
<br class="">
</div>
</div>
</blockquote>
</span>
</body>
</html>