<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Gongpu,<div class=""><br class=""></div><div class="">Indeed pressure should go up to around 2050 bar.</div><div class=""><br class=""></div><div class="">A few things to check (which you probably already did).</div><div class="">If you turn the blue knob on the side you can adjust the pressure, try adjusting that.</div><div class="">Is the incoming pressure from the compressor OK (about 10bar)?</div><div class=""><br class=""></div><div class="">Let me know if it works (or not), otherwise I can think of a few other things to try / check.</div><div class=""><br class=""></div><div class="">Good luck,</div><div class="">Paul</div><div class=""><div apple-content-edited="true" class=""><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="" style="word-wrap: break-word; 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-webkit-line-break: after-white-space;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><div class="">__________________________________</div><div class="">Paul Verkade PhD FRMS</div><div class="">Reader in Cell Imaging</div><div class="">Wolfson Bioimaging Facility</div><div class="">Schools of Biochemistry and Physiology & Pharmacology</div><div class="">Biomedical Sciences Building</div><div class="">University of Bristol</div><div class="">University Walk</div><div class="">BS8 1TD Bristol</div><div class="">United Kingdom</div><div class=""><br class="khtml-block-placeholder"></div><div class="">tel.+44-117-3312179</div><div class="">Fax. +44-117-3312168</div><div class="">mail: <a href="mailto:p.verkade@bristol.ac.uk" class="">p.verkade@bristol.ac.uk</a></div><div class="">websites:</div><div class=""><a href="http://www.bristol.ac.uk/biochemistry/research/pv.html" class="">http://www.bristol.ac.uk/biochemistry/research/pv.html</a></div><div class=""><a href="http://www.bris.ac.uk/phys-pharm/research/staffresearch/paulverkade.html" class="">http://www.bris.ac.uk/phys-pharm/research/staffresearch/paulverkade.html</a></div><div class=""><a href="http://www.bristol.ac.uk/biochemistry/wbif" class="">http://www.bristol.ac.uk/biochemistry/wbif</a></div><div class=""><br class=""></div></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></div></span></span></span></div></span></div></span></div></span></div></span></div></span></div></div></span></div></span></div></span></div></span></div></div><div><blockquote type="cite" class=""><div class="">On 6 Oct 2015, at 20:42, Zhao, Gongpu <<a href="mailto:goz1@pitt.edu" class="">goz1@pitt.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Dear All,<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">We are experiencing a pressure failure problem during high pressure freezing using Leica EM Pact . For successful freezing, the curve is smooth and pressure should go up to 2000 bar. But right now, the curve is fuzzy, at some points it drops and the small display shows the pressure is failure. I have attached an image of the bad curve. Any insights about what might cause the problem and how to fix it would be greatly appreciated? Thank you very much.<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">gongpu<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">GONGPU ZHAO, PH.D.<o:p class=""></o:p></span></b></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">Research Associate<o:p class=""></o:p></span></i></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">Department of Structural Biology<o:p class=""></o:p></span></i></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">University of Pittsburgh School of Medicine<o:p class=""></o:p></span></i></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><i class=""><span style="font-family: 'Times New Roman', serif; color: rgb(0, 112, 192);" class="">3501 5th Ave. RM2054, Pittsburgh, PA 15260<o:p class=""></o:p></span></i></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div></div><span id="cid:0EA97C78-4396-4082-9A86-DAAA2062C862@users.bris.ac.uk"><pressure_failure.jpg></span><span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">3dem mailing list</span><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></div></blockquote></div><br class=""></body></html>