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<p class="MsoNormal">Dear All,<o:p></o:p></p>
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<p class="MsoNormal">We are experiencing a pressure failure problem during high pressure freezing using Leica EM Pact . For successful freezing, the curve is smooth and pressure should go up to 2000 bar. But right now, the curve is fuzzy, at some points it
drops and the small display shows the pressure is failure. I have attached an image of the bad curve. Any insights about what might cause the problem and how to fix it would be greatly appreciated? Thank you very much.<o:p></o:p></p>
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<p class="MsoNormal">gongpu<o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-family:"Times New Roman","serif";color:#0070C0">GONGPU ZHAO, PH.D.<o:p></o:p></span></b></p>
<p class="MsoNormal"><i><span style="font-family:"Times New Roman","serif";color:#0070C0">Research Associate<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-family:"Times New Roman","serif";color:#0070C0">Department of Structural Biology<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-family:"Times New Roman","serif";color:#0070C0">University of Pittsburgh School of Medicine<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-family:"Times New Roman","serif";color:#0070C0">3501 5th Ave. RM2054, Pittsburgh, PA 15260<o:p></o:p></span></i></p>
<p class="MsoNormal"><o:p> </o:p></p>
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