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Dear all,
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<div>I am a new student to the field of electron microscopy. As a part of a project to an electron microscopy course I am taking, we are trying to look at negatively stained ribosomes. Does anyone have experience regarding this? Buffers used, concentrations,
stain used? I did search the literature few times to look for recent publications on the topic but didn’t come across anything useful. I started with 2% Uranyl Acetate in “20 mM HEPESKOH (pH 7.5), 6 mM MgCl<span style="vertical-align: -1pt;">2</span>, 150
mM NH<span style="vertical-align: -1pt;">4</span>Cl, 6 mM b-mercaptoethanol, 2 mM spermidine, and 0.1 mM spermine” with a concentration of 15 nM. </div>
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<div>Bassem Shebl<br>
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