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<p class="MsoNormal">Hi Everyone,<o:p></o:p></p>
<p class="MsoNormal">We are about to embark on some cryo fib milling of cells grown on grids with a view to do cryo tomography. The plan is to use a FEI Quanta 3D FEG fitted with a Gatan ALTO cryo system. I have seen data using this technique but using e.g.
a Polara (Peijun Zhang). Has anyone tried to fabricate holders to take FEI Titan cartridges into a cryo FIB (and out again) and successfully load them into the cassette. We will need to mill the cartridges to allow for a shallow milling angle but that has
been shown before by Peijun and colleagues.<o:p></o:p></p>
<p class="MsoNormal">I have concerns about the delicate handling of the cartridges to get them in and out of a shuttle system and then into the cassette. Autoloaders are not tolerant of any excess ice.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">If anyone has experience or thoughts I would be most grateful to hear from them.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Regards<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Chris<o:p></o:p></p>
<p class="MsoNormal"><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Christopher J. Gilpin Ph.D.<o:p></o:p></p>
<p class="MsoNormal">Director, Life Science Microscopy Facility<o:p></o:p></p>
<p class="MsoNormal">Purdue University<o:p></o:p></p>
<p class="MsoNormal">Whistler Hall of Agriculture Research, Room S052<o:p></o:p></p>
<p class="MsoNormal">170 S. University St<o:p></o:p></p>
<p class="MsoNormal">West Lafayette, IN 47907 <o:p></o:p></p>
<p class="MsoNormal">765-494-7750<o:p></o:p></p>
<p class="MsoNormal"><a href="mailto:gilpin@purdue.edu"><span style="color:blue">gilpin@purdue.edu</span></a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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