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Dear all,<br>
<br>
thank you very much for your replies. All your answers were very
helpful to me.<br>
<br>
Best,<br>
Andreas<br>
<br>
<div class="moz-cite-prefix">On 01/28/2015 10:22 PM, Brink Jaap
wrote:<br>
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<blockquote cite="mid:8A7B2FEF-A21D-4B3B-9F52-9CE95F66DC51@jeol.com"
type="cite">
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Hi,
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<div class="">There ought to be a set of workshop drawings
floating around on the internet for a simple plunger that Tim
Baker was using when at Purdue (not sure what he uses now).
Anyways, this plunger has al the features of a cheap but very
effective plunger you’d need. Comes with footpads release …. If
you can’t find them, drop me a note off-line.</div>
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<div class="" style="text-align:
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<blockquote type="cite" class="">
<div class="">On Jan 28, 2015, at 2:58 PM, <a
moz-do-not-send="true"
href="mailto:benoit.zuber@ana.unibe.ch" class="">benoit.zuber@ana.unibe.ch</a>
wrote:</div>
<br class="Apple-interchange-newline">
<div class="">Hi Andrea,<br class="">
<br class="">
Depending on what you want to do, you could also have your
mechanical workshop build one plunger for less than hundred
euros. It needs an arm that you can attach tweezers on. The
arm needs to be heavy so that it accelerates well during
free fall. It needs to be long enough to reach a sufficient
speed when the grid will touch ethane. And it needs a
release mechanism triggered either by hand or preferably by
a foot pedal if you want to hold the blotting paper with
both hands. To prevent the cryogen to get frozen, you can
use a mixture of Ethane and propane, have small resistance
in the ethane container, or have the container in contact
but not fully immersed in liquid nitrogen. That way ethane
is not cold enough to freeze but it is cold enough to
vitrify water. You can check with a thermometer that the
temperature is correct before starting to plunge. We use
such a plunger most of the time and are absolutely
delighted. Never a software bug or any blocked pad, or
expensive maintenance contract/Cost. whatever other problem.
It takes a bit of practice at the beginning to get the
feeling for the blotting in order to get the right ice
thickness but after a few sessions you will have it and just
like riding a bike, you will not forget it. Glow discharging
the grid in amyl amine atmosphere is said to help against
evaporation and therefore get reproducible ice thickness (I
used to do that but we don't anymore). Some people will tell
you that controlling humidity is absolutely critical, that
the grids will dry otherwise. I do not exclude that there
are cases where humidity control matters but quite often it
does not. If this is a concern you can anyway get a
humidifier from the local supermarket and put the plunger in
a rather closed and chilled hood (taking care though not to
ignite ethane)!<br class="">
<br class="">
Best <br class="">
Benoît <br class="">
<br class="">
<br class="">
<br class="">
Le 28 janv. 2015 à 18:22, Andreas <<a
moz-do-not-send="true"
href="mailto:andreas.schmidt@uni-ulm.de" class="">andreas.schmidt@uni-ulm.de</a>>
a écrit :<br class="">
<br class="">
Dear all,<br class="">
<br class="">
I am a PhD student at the University of Ulm, Germany and our
Institute wants to buy another cryo plunger. We already
bought a Gatan CP3 and now I was asked to look for other
commercial available cryo plungers. So far I also found the
Vitrobot by FEI, the EMS-002 Rapid Immersion Freezer by EMS
and the Leica EM GP by Leica. Are there any other companies
selling cryo plungers and would you recommend them? Or is
there anyone who would like sell their old device?<br
class="">
<br class="">
<br class="">
Best regards,<br class="">
Andreas<br class="">
<br class="">
-- <br class="">
Andreas Schmidt<br class="">
Biochemist<br class="">
Institute for Pharmaceutical Biotechnology<br class="">
Ulm University<br class="">
Helmholtzstrasse 8/1<br class="">
89081 Ulm<br class="">
Germany<br class="">
<br class="">
Tel: +49 (0)731 503 2767<br class="">
Email: <a moz-do-not-send="true"
href="mailto:andreas.schmidt@uni-ulm.de" class="">andreas.schmidt@uni-ulm.de</a><br
class="">
<br class="">
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<br>
<pre class="moz-signature" cols="72">--
Andreas Schmidt
Biochemist
Institute for Pharmaceutical Biotechnology
Ulm University
Helmholtzstrasse 8/1
89081 Ulm
Germany
Tel: +49 (0)731 503 2767
Email: <a class="moz-txt-link-abbreviated" href="mailto:andreas.schmidt@uni-ulm.de">andreas.schmidt@uni-ulm.de</a></pre>
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