<div dir="ltr"><div><div><div>Dear Sheemei,<br><br>These are due to the characteristics of the protein to stick to carbon. We faced similar problem and one trick to overcome this problem is<br></div>use a thin layer are carbon on the holes of holey grid. Another advantage is you can reduce the concentration of sample required to a large<br>extent; you can try almost 1/10th that you use in holey grids.<br> Use of plasma treated graphene is another method that you can try - see recent Nat. Methods paper by Lori and group "Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas. Russo CJ, Passmore LA. Nat Methods. 2014 Jun;11(6):649-52.<br><br>Hope that helps,<br>Warm Regards,<br></div><div>Natesh<br></div></div><div class="gmail_extra"><br>- <br><div class="gmail_signature"><div dir="ltr">----------------------------------------------------------<br>"Live Simply and do Serious Things .. "<br>- Dorothy Mary Crowfoot Hodgkin OM, FRS<br><br>"In Science truth always wins"<br>- Max Ferdinand Perutz OM FRS<br>----------------------------------------------------------<br>Dr. Ramanathan Natesh<br>Ramalingaswami Fellow-DBT, Assistant Professor<br>Indian Institute of Science Education and Research Thiruvananthapuram (IISER-TVM),<br><div>
1st floor, Dept. of Computer Science & Engg. Building,<br>CET Campus, Engineering College P.O.,<br></div>Trivandrum, 695016, Kerala, India<br><br><a href="mailto:natesh@iisertvm.ac.in" target="_blank">natesh@iisertvm.ac.in</a><br><a href="http://www.researcherid.com/rid/C-4488-2008" target="_blank">http://www.researcherid.com/rid/C-4488-2008</a><br><a href="http://iisertvm.ac.in/%7Enatesh" target="_blank">http://iisertvm.ac.in/~natesh</a><br>Ph. 0091- 471-2599403<br>Fax.0091-471-2597427</div></div><br><div class="gmail_quote">On 31 October 2014 11:28, Sheemei Lok <span dir="ltr"><<a href="mailto:sheemei.lok@duke-nus.edu.sg" target="_blank">sheemei.lok@duke-nus.edu.sg</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div style="word-wrap:break-word;color:rgb(0,0,0);font-size:14px;font-family:Calibri,sans-serif">
<div>Dear all,</div>
<div><span style="white-space:pre-wrap"></span>Our protein sample tend to stick to the carbon film instead of the hole in quantifoil. Are there any tricks that we can do to get them into the holes?</div>
<div><br>
</div>
<div>Regards,</div>
<div>
<div><span style="font-family:Calibri,Verdana,Helvetica,Arial;font-size:medium"><span style="font-size:11pt">Shee-Mei Lok<br>
</span><font size="1"><span style="font-size:9pt"><i>Associate Professor<br>
Program in Emerging Infectious Diseases,<br>
Duke-NUS Graduate Medical School,<br>
KTP Building<br>
Level 9 Room 19<br>
8 college Road,<br>
Singapore 169857<br>
Ph: 65-65165840<br>
Fax:65-62212529<br>
email: <u><a>sheemei.lok@duke-nus.edu.sg</a></u></i></span></font><span style="font-size:11pt"> <br>
</span></span>
<div><br>
</div>
<div><br>
</div>
</div>
</div>
<div><br>
</div>
<div><br>
</div>
<br>
<hr>
<font color="Black" face="Arial" size="1"><br>
Important: This email is confidential and may be privileged. If you are not the intended recipient, please delete it and notify us immediately; you should not copy or use it for any purpose, nor disclose its contents to any other person. Thank you.<br>
</font>
</div>
<br>_______________________________________________<br>
3dem mailing list<br>
<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
<a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>
<br></blockquote></div><br><br clear="all"><br>-</div></div>