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NIH</title></head><body>
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<div><font size="-1" color="#000000"><b>Staff Scientist Position
Available<br>
Laboratory of Cell Biochemistry and Biology<br>
National Institute of Health, USA<br>
<br>
</b>A staff scientist position is available in the laboratory of Jenny
Hinshaw at NIH. The qualified candidate may participate in
ongoing research projects of the Hinshaw laboratory and/or
initiate independent research projects in closely related fields. The
successful candidate should have a Ph.D. with postdoctoral experience
in Cell and Structural biology involving cryo-EM and image
processing. </font><br>
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<div><font size="-1" color="#000000">A major focus of our research is
calculating 3 dimensional structures of proteins involved in
endocytosis and membrane remodeling events. We currently have solved
the structure of a dynamin mutant in the constricted and
non-constricted states to a resolution of 20 Å. Dynamin is
crucial for endocytosis,<i> synaptic</i> membrane recycling, membrane
trafficking within the cell and more recently has been associated with
filamentous actin. Other dynamin family members are involved in
mitochondria division and morphology (Drp1, Mgm1), anti-viral activity
(Mx, hGBP1), and plant cell wall formation and thylakoid
membrane organization (Phragmoplastin, ADLs). To determine if a common
mechanism exists among this unique set of proteins, we plan to
calculate and compare the 3D structure of dynamin family members using
cryo-electron microscopy, helical reconstruction and single
particle methods. Another interest of the laboratory is to
determine the 3-dimensional structure of the nuclear pore complex
(NPC) in<i> C. Elegans</i>. This project will entail developing
a method of isolating NPCs from<i> C. Elegans</i> and comparing
various NPC mutants using the<i> C. Elegans</i> well-developed genetic
system. The 3D structure of<i> Xenopus</i> NPC was previously
solved by Drs. Hinshaw, Carragher and Milligan and will be used as a
model system for developing the<i> C. Elegans</i>
system. </font></div>
<div><font size="-1" color="#000000"><br>
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Contact: <b> Jenny E. Hinshaw<br>
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</b>Laboratory of Cell Biochemistry and Biology<x-tab>
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NIDDK, NIH<x-tab>
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Building 8, Room 419<x-tab>
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8 Center Dr MSC 0851<x-tab>
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Bethesda, MD 20892, USA<br>
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<i>phone</i>: +1 301 594 0842<x-tab> </x-tab><br>
<i>fax</i>: +1 301 496 9431<br>
<i>e-mail</i>:<u>
jennyh@helix.nih.gov</u><x-tab>
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http://dynamin.niddk.nih.gov/</font></div>
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