<HTML><BODY style="word-wrap: break-word; -khtml-nbsp-mode: space; -khtml-line-break: after-white-space; "><DIV><BR class="khtml-block-placeholder"></DIV><DIV>Hi,</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>We are happy to announce the first release of the 2dx software. </DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>2dx provides a graphical user interface for the computer processing of 2D crystal images. So far, the program 2dx_image is available for the processing of individual images of tilted or non-tilted 2D crystals. Merging will be implemented in the near future.</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>2dx assists running c-shell scripts that call other stand-alone programs. In the current release we provide a set of MRC programs, which were slightly adapted to interact with the 2dx software, and we added a few functions trying to implement automatic determination of all required parameters. It is easy to adapt or replace the provided scripts with your own scripts and/or to make use of other back-end programs (Spider, bsoft, etc.) or your own MRC software. </DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV>2dx including the source codes is freely available, and we provide compiled versions for Linux x86_64bit and Mac-PPC (G4 (slow), G5) and the new Mac-Intel computers, see here:</DIV><DIV><A href="http://2dx.org">http://2dx.org</A></DIV><DIV style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">The installer hopefully take care of everything (all goes into /usr/local/2dx), and installs also the required modified MRC programs. The only thing that is required in addition is CCP4, as described on the server. But CCP4 is only used in the last script for the generation of a map. The rest should also work without CCP4. </DIV><DIV style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">On the server are also some test data, in MRC-image2000 file format for big and small endian machines, together with the corresponding config files (2dx_image.cfg).</DIV><P style="margin: 0.0px 0.0px 0.0px 0.0px"> </P><DIV style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">A manuscript that describes this software is under review at JSB, but we don't have written the manual for 2dx yet. However, hopefully, most features in 2dx are self-explanatory. Just try the right mouse button over all the different items, or double-clicking.</DIV><DIV style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Helvetica; min-height: 14px; "><BR></DIV><DIV style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Bryant, Xiangyan and Henning.</DIV><P style="margin: 0.0px 0.0px 0.0px 0.0px"> </P><DIV><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><SPAN class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><DIV><BR></DIV><DIV>Henning Stahlberg,</DIV><DIV>Molecular & Cellular Biology, Briggs Hall 5,</DIV><DIV>University of California at Davis, 1 Shields Ave., Davis, CA 95616, USA </DIV><DIV>Tel: +1-530-752 8282 (office), +1-530-754 8285 (lab) Fax: +1-530-752 3085 </DIV><DIV><A href="mailto:HStahlberg@ucdavis.edu">mailto:HStahlberg@ucdavis.edu</A> <A href="http://stahlberglab.ucdavis.edu">http://stahlberglab.ucdavis.edu</A> </DIV><DIV><A href="AIM:HStahlberg@mac.com">AIM:HStahlberg@mac.com</A></DIV><DIV><BR></DIV><DIV>Workshop on Electron Crystallography, August 7-11, 2006, at UC Davis.</DIV><DIV><A href="http://2dx.org/workshop">http://2dx.org/workshop</A></DIV><DIV>_____________________________________________________________</DIV><DIV><BR class="khtml-block-placeholder"></DIV><DIV><BR class="khtml-block-placeholder"></DIV><BR class="Apple-interchange-newline"></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN></SPAN> </DIV><BR></BODY></HTML>