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Dear all<br><br>
We are experiencing some problems to obtain good vitrification of
protein samples purified in Hepes buffer (The ice contains some funny
looking "strings").<br>
These problems seem to disappear when dialyzing the sample,<br>
but our protein is not very happy in other standard buffers like PBS or
Tris.<br><br>
Does anyone know if there is a real problem in using Hepes buffer for
cryoEM, or might we just be having a different problem we still don´t
know?<br><br>
Thanks for the help<br>
Oscar Llorca <br><br>
<br><br>
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<div align="center"><b>Dr Oscar Llorca<br>
Centro de Investigaciones Biológicas-CSIC<br>
</b><i>Centre for Biological Research<br><br>
</i>Ramiro de Maeztu, 9<br>
28040 Madrid (Spain)<br><br>
<br>
Group Web Site:
<a href="http://electronmicroscopy.cib.csic.es/" eudora="autourl">http://electronmicroscopy.cib.csic.es</a><br><br>
E-mail: ollorca@cib.csic.es<br>
Tel: +-34-91-837 3112 ext 4446<br>
Fax: +-34-91-536 04 32<br><br>
<br><br>
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