<!doctype html public "-//W3C//DTD W3 HTML//EN">
<html><head><style type="text/css"><!--
blockquote, dl, ul, ol, li { padding-top: 0 ; padding-bottom: 0 }
--></style><title>Post-Doctoral position</title></head><body>
<div>Dear colleagues,</div>
<div><br></div>
<div>A two years post-doctoral position is available in our laboratory
to study the interaction between CLIP-170 and microtubules using new
fluorescent probes, the Quantum-Dots</div>
<div><br></div>
<div>Microtubules are a major component of the cytoskeleton in
eukaryotic cells where they are essential for cell morphogenesis,
intracellular trafficking and cell division. Their dynamic properties
are finely regulated by a variety of proteins such as MAPs
(Microtubule Associated Proteins) or "catastrophe" and "rescue"
factors. Among these proteins, cytoplasmic linker protein CLIP-170 was
identified in cells at growing microtubule plus ends where it
modulates their dynamics and their interactions with cellular
organelles. Recently, we showed that CLIP-170 is a rescue factor that
interacts with tubulin in solution and induces the formation of curved
oligomers (Arnal et al., 2004, Curr. Biol., 14:2086). These oligomers
are assembly intermediates, a behavior that accounts for the targeting
of CLIP-170 at microtubule ends. We proposed that the straightening of
the oligomers that occurs during microtubule wall formation may induce
CLIP-170 release, and thus would restrict it to the growing end of
microtubules. To test this model, we plan to label CLIP-170 with
fluorescent nanoparticules (Quantum-Dots), which present a high
photostability and are also visible by electron microscopy. The
labeled protein (CLIP-170*) will be followed in real time using
video-light microscopy on microtubules assembled from pure tubulin and
centrosomes. Its precise localization on the various assembly
intermediates and on microtubules will be investigated using
cryo-electron microscopy and image analysis (3D
reconstructions).</div>
<div><br>
The successful candidate will have experience in video-light
microscopy and/or cryo-electron microscopy. Experience in protein
purification and labeling will be appreciated.</div>
<div><br></div>
<div>The starting date is September 2005.<font color="#000000">
Details can be found at
http://www.umr6026.univ-rennes1.fr/sdm/</font></div>
<div><font color="#000000">Apply by sending a CV, names of two
referees and application letter to Dr. Denis Chrétien :
denis.chretien@univ-rennes1.fr</font></div>
<div><font color="#FF0000"><br></font></div>
<div><font color="#FF0000">_____________________________________<br>
Denis Chretien</font></div>
<div><font color="#FF0000">Equipe "Structure et Dynamique des
Macromolécules"<br>
UMR 6026 "Interactions Cellulaires et Moleculaires"<br>
Universite de Rennes 1<br>
Campus de Beaulieu, Bt 13<br>
35042 RENNES Cedex<br>
FRANCE<br>
_____________________________________</font></div>
<div><font color="#FF0000">tel: +33 (0) 2 23 23 67 64<br>
+33 (0) 6 62 00 84 57<br>
fax: +33 (0) 2 23 23 50 48<br>
email: denis.chretien@univ-rennes1.fr</font></div>
<div><font
color="#FF0000">http://www.umr6026.univ-rennes1.fr/sdm/</font></div>
<div><font
color="#FF0000">_____________________________________</font></div>
</body>
</html>