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<title>RE: [3DEM] A question about ice thickness of cryo-EM</title>
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<p class=MsoNormal><font size=4 color=navy face="Times New Roman"><span
style='font-size:14.0pt;color:navy'>Hi Paul,</span></font></p>
<p class=MsoNormal><font size=4 color=navy face="Times New Roman"><span
style='font-size:14.0pt;color:navy'> </span></font></p>
<p class=MsoNormal style='line-height:150%'><font size=4 color=navy
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
color:navy'>Thanks for your information! You might have given me a basic idea
what the maximum of ice thickness could be. Actually as indicated in my
earliest message, I’m not trying to figure out how thick the ice could be
in any specific case. What I’m trying to find out is an estimate of the maximum
of ice thickness of cryo-EM grids which is good enough for imaging. I wonder if
anybody has ever collected data with ice thicker than 800 nm. </span></font></p>
<p class=MsoNormal style='line-height:150%'><font size=4 color=navy
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
color:navy'>Thanks for any further help!</span></font></p>
<p class=MsoNormal style='line-height:150%'><font size=4 color=navy
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
color:navy'> </span></font></p>
<p class=MsoNormal style='line-height:150%'><font size=4 color=navy
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
color:navy'>Regards,</span></font></p>
<p class=MsoNormal style='line-height:150%'><font size=4 color=navy
face="Times New Roman"><span style='font-size:14.0pt;line-height:150%;
color:navy'>Guobin</span></font></p>
<p class=MsoNormal><font size=4 color=navy face="Times New Roman"><span
style='font-size:14.0pt;color:navy'> </span></font></p>
<p class=MsoNormal><font size=4 color=navy face="Times New Roman"><span
style='font-size:14.0pt;color:navy'> </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'> </span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> owner-3dem@ncmir.ucsd.edu
[mailto:owner-3dem@ncmir.ucsd.edu] <b><span style='font-weight:bold'>On Behalf
Of </span></b>Paul Chipman<br>
<b><span style='font-weight:bold'>Sent:</span></b> </span></font><font size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>Thursday,
February 17, 2005</span></font><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'> </span></font><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>10:50 AM</span></font><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'><br>
<b><span style='font-weight:bold'>To:</span></b> 3dem@ucsd.edu<br>
<b><span style='font-weight:bold'>Subject:</span></b> RE: [3DEM] A question
about ice thickness of cryo-EM</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Hi Guobin,</span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
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<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>The ice thickness is a product of the
environmental conditions (humidity, temperature), the composition of the
sample, the blotting duration, pressure and paper and also the characteristics
of the grid. I don't think the grid hole size or periodicity alone
dictates your ice thickness. With the Quantifoil 7/2 we can get vitreous
ice that ranges from ~25-800nm. And, as Yifan mentioned, we can also get
ice too thick to image through.</span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Cheers,</span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Paul</span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
</div>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'>Hi Yifan,</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'>Thanks for your response. You brought a really good
suggestion. I love to try it. However due to our very limited access to the
scope and due to the fact that we don't have a CCD camera, I prefer to get a
direct answer to the question from our 3DEM community. I guess some people do
have experience on this issue.</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'>By the way, as indicated in my previous message, the
purpose for me to estimate the maximum of ice thickness is for my image
analysis, which implied that the ice thickness is still appropriate for
imaging. On the other hand, my experience suggested that ice thickness varies
on different type of grids, for example, on 7/2 quantifoil holy grids, I
usually get thinner ice than that on 3.5/1 quantifoil holy grids. But I have
never tried to figure out how significant the difference of ice thickness could
be.</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'>I'm still waiting for more response and an answer.
Thanks for attention and help!</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'>Regards,</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'>Guobin</span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=4 face="Times New Roman"><span
style='font-size:13.5pt'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>-----Original Message-----<br>
From: Yifan Cheng [mailto:Yifan_Cheng@hms.harvard.edu]<br>
Sent: </span></font><font size=2 face="Courier New"><span style='font-size:
10.0pt;font-family:"Courier New"'>Tuesday, February 15, 2005</span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>
</span></font><font size=2 face="Courier New"><span style='font-size:10.0pt;
font-family:"Courier New"'>6:39 PM</span></font><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'><br>
To: Hu, Guobin ; 3dem@ucsd.edu<br>
Subject: Re: [3DEM] A question about ice thickness of cryo-EM</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Hi</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Well, the ice can be very
very very thick no matter what grids you use.</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Did you ever get cryo grids
which is too thick to be seen through?</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>What you can do to measure
the ice thickness is to burn a small hole in</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>the middle of the ice. Then
tilt you holder to a high tilt say 60 degree</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>and measure the size of the
hole. This can tell you how thick is your</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>ice. Then you can calibrate
it with your CCD camera by measure the</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>counts over an empty hole
and over ice. Next time just by comparing the</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>CCD counts over ice and
empty hole will give you some fairly good</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>estimation about your ice
thickness. Good luck</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Yifan</span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Hu, Guobin wrote:</span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> Hi colleagues,</span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>></span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> For our image analysis,
we need to estimate the maximum ice thickness in</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> our cryo-EM specimen on
3.5/1 quantifoil grids. We may be able to</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> estimate that based on
the thickness of carbon foil. As indicated by the</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> quantifoil product
catalogue, the standard thickness of carbon foil is</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> 12 nm. While
observations of our cryo-EM micrographs have suggested that</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> the thickness of carbon
foil is definitely much larger than 12 nm. Does</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> anybody have an idea of
what the maximum thickness of ice could be on</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> quantifoil grids
(3.5/1)?</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>></span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> Thanks for help!</span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> Guobin Hu</span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>> </span></font></p>
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style='font-size:10.0pt;font-family:"Courier New"'>> </span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>--</span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>************************************************************</span></font></p>
</blockquote>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Yifan Cheng, Ph.D.</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Department of Cell Biology, </span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Harvard</span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>
</span></font><font size=2 face="Courier New"><span style='font-size:10.0pt;
font-family:"Courier New"'>Medical</span></font><font size=2
face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'> </span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>School</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>240 Longwood Avenue, </span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Boston</span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>,
</span></font><font size=2 face="Courier New"><span style='font-size:10.0pt;
font-family:"Courier New"'>MA</span></font><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>02115</span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>,
</span></font><font size=2 face="Courier New"><span style='font-size:10.0pt;
font-family:"Courier New"'>USA</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Phone: 1-617-432-4095
(office), 432-4097 (wet lab),</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>
432-4708 (EM lab), 432-4107 (computer lab);</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Fax:
1-617-432-1144 (department)</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>email:
Yifan_Cheng@hms.harvard.edu</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>************************************************************</span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'> </span></font></p>
</blockquote>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite cite>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
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<pre style='margin-left:.5in'><font size=2 face="Courier New"><span
style='font-size:10.0pt'><x-sigsep>-- </span></font></pre></x-sigsep>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=4 color=black
face="Courier New"><span style='font-size:13.5pt;font-family:"Courier New";
color:black'>Paul Chipman<br>
Electron Microscopy Facility Manager<br>
Dept. of Biology, </span></font><font size=4 color=black face="Courier New"><span
style='font-size:13.5pt;font-family:"Courier New";color:black'>Purdue</span></font><font
size=4 color=black face="Courier New"><span style='font-size:13.5pt;
font-family:"Courier New";color:black'> </span></font><font size=4
color=black face="Courier New"><span style='font-size:13.5pt;font-family:
"Courier New";color:black'>University</span></font><font size=4 color=black
face="Courier New"><span style='font-size:13.5pt;font-family:"Courier New";
color:black'><br>
Lilly Hall, Rm. B216<br>
Phone: 765-494-1487<br>
Fax:765-496-1189</span></font></p>
</div>
</blockquote>
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