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<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Dear Ulrich, Roman, Joel,<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p>&nbsp;</o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Thanks for your suggestion. I still do not
know if our problem is due to the misadjustment.<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>It happens sometimes and does not at the
other time. It happened both for commercial<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>and handmade grids (I have not tried
further carbon coating). But, we are happy if FEI<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>engineers take a look at our problem for
us (so, please either let them know about that<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>or have a look by yourself, Joel).<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p>&nbsp;</o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Best wishes,<o:p></o:p></span></font></p>

<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Takashi<o:p></o:p></span></font></p>

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<p><font size=2 color=navy face=Arial><span style='font-size:10.0pt;font-family:
Arial;color:navy'>Takashi Ishikawa</span></font><font color=navy><span
style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>Research Scientist / Group Leader</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>Department of Biology</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>Swiss Federal Institute of Technology (ETH
Zürich)</span></font><font color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>ETH Hönggerberg, HPK F7</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>CH-8093 Zürich, Switzerland</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>phone: +41 1-633-2462</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>Fax:&nbsp; : +41 1-633-1073</span></font><font
color=navy><span style='color:navy'> <br>
</span></font><font size=2 color=navy face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:navy'>e-mail: <st1:PersonName w:st="on">takashi.ishikawa@mol.biol.ethz.ch</st1:PersonName></span></font><font
color=navy><span style='color:navy'> </span></font><o:p></o:p></p>

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<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> Joel Paul
[mailto:joelpaul@sbcglobal.net] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, February 09, 2005
1:19 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Ishikawa, Takashi<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [3DEM] Gatan
cryopluge</span></font><o:p></o:p></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p>&nbsp;</o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Book Antiqua"><span style='font-size:
12.0pt;font-family:"Book Antiqua"'>Takashi,</span></font><o:p></o:p></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>&nbsp;<o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Book Antiqua"><span style='font-size:
12.0pt;font-family:"Book Antiqua"'>I'm the Vitrobot product engineer in <st1:place
w:st="on">North America</st1:place>. Reading your post it would seem that you
have a problem with your blot assembly not closing properly. I have seen this
in <st1:country-region w:st="on"><st1:place w:st="on">America</st1:place></st1:country-region>
at a few sites and we have identified this as an adjustment that needs to be
done. There is a grub screw at the back of the assembly that will become loose
after six months usually. Can your local service engineer make a call to the
lab to investigate the issue? If this is not possible I will be in <st1:place
w:st="on">Europe</st1:place> next week and could possibly come to the lab.</span></font><o:p></o:p></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>&nbsp;<o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Book Antiqua"><span style='font-size:
12.0pt;font-family:"Book Antiqua"'>Regards,</span></font><o:p></o:p></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>&nbsp;<o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Joel Paul<o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>FEI Company<o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Sr. Technical Support Engineer<o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>TEM TSG <st1:place w:st="on">North America</st1:place><o:p></o:p></span></font></p>

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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>&nbsp;<font color=navy><span style='color:navy'><o:p></o:p></span></font></span></font></p>

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