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<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Bill;</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> We
have had the same problem for a couple of times already in our new Polara G2 in
the last couple of months. We were told by FEI an engineer that this was due to
the different temperatures between the stage and the insertion rod. Their
reasoning is that if you insert a cartridge at room temperature (carbon film
test specimen) into a cooled column (liquid nitrogen), the cartridge will be "frozen"
to the stage and it won't release even after unscrewing. I could not possibly
figure out how a dry copper cartridge can possibly be frozen to a metal surface
the high vacuum of the column and was not convinced by their explanation. Nor
do I have an explanation myself. </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> I
should indicate that during one of the two incidences we had, the problem
mysteriously disappeared after leaving the cartridge in the stage overnight. </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Hong</span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> </span></font></p>
<p class=MsoNormal style='layout-grid-mode:char'><u><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'>_________________________________________________________</span></font></u></p>
<p class=MsoNormal style='layout-grid-mode:char'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'>Z. Hong Zhou,
Ph.D., Assoc. Prof., <a href="http://hub.med.uth.tmc.edu/~hong">http://hub.med.uth.tmc.edu/~hong</a>
</span></font></p>
<p class=MsoNormal style='layout-grid-mode:char'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'>Ph:
713.500.5358 Fax:713.500.0730, Email: <a
href="mailto:Z.H.Zhou@uth.tmc.edu">Z.H.Zhou@uth.tmc.edu</a> </span></font></p>
<p class=MsoNormal style='layout-grid-mode:char'><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'>University</span></font><font
color=black><span style='color:black'> of </span></font><font color=black><span
style='color:black'>Texas</span></font><font color=black><span
style='color:black'> – Houston, </span></font><font color=black><span
style='color:black'>Medical</span></font><font color=black><span
style='color:black'> </span></font><font color=black><span style='color:black'>School</span></font><font
color=black><span style='color:black'>, </span></font><font size=1 color=black><span
style='font-size:9.0pt;color:black'>6431 Fannin, MSB2.280</span></font></p>
<p class=MsoNormal style='layout-grid-mode:char'><u><font size=3 color=black
face="Times New Roman"><span style='font-size:12.0pt;color:black'>Department of
Pathology & Laboratory Medicine, </span></font></u><u><font color=black><span
style='color:black'>Houston</span></font></u><u><font color=black><span
style='color:black'>, </span></font></u><u><font color=black><span
style='color:black'>TX</span></font></u><u><font color=black><span
style='color:black'> </span></font></u><u><font color=black><span
style='color:black'>77030</span></font></u></p>
<p class=MsoNormal style='layout-grid-mode:char'><strong><b><font size=1
color=black face=Arial><span style='font-size:9.0pt;font-family:Arial;
color:black'>Secretary support:</span></font></b></strong><font color=black><span
style='color:black'> </span></font><font size=1 color=black><span
style='font-size:9.0pt;color:black'>Ms Chris Haverkorn 7135005316 <a
href="mailto:Christine.M.Haverkorn@uth.tmc.edu">Christine.M.Haverkorn@uth.tmc.edu</a></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"> </font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>-----Original Message-----<br>
From: owner-3dem@ncmir.ucsd.edu [mailto:owner-3dem@ncmir.ucsd.edu] On Behalf Of
Bill Tivol<br>
Sent: </span></font>Wednesday, October 13, 2004 7:37 PM<br>
To: 3dem@ucsd.edu<br>
Subject: [3DEM] F30 cartridge problem</p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>**** Messages to this list are automatically archived
***</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>**** Please limit quoting of previous postings to the
bare minimum ****</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Dear List,</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> Lately
we have been having trouble when trying to extract a cartridge </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>from the stage into the insertion rod. The
cartridge stays in the </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>stage after the thread has been unscrewed. This
has occurred with </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>cartridges of all types--screw ring, c-clip, and
flip-flop. The only </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>way we know that works to extract successfully is to
put hot water in </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>the dewar on the insertion rod and heat the rod to ~50
C. Even this </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>method is not always successful, and, of course, if we
wish to continue </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>studying a frozen-hydrated specimen, such as when
taking a dual-axis </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>tilt series, such a warm-up is unacceptable. Has
anyone 1) seen this </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>problem, 2) managed to determine the cause, and/or 3)
found a way to </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>extract the cartridge that does not spoil the
specimen? TIA for any </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>help.</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Yours,</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Bill Tivol, PhD</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>EM Scientist and Manager</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Cryo-Electron Microscopy Facility</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Broad</span></font> Center, Mail Code 114-96</p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>California Institute of Technology</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Pasadena</span></font> CA 91125</p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>(626) 395-8833</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>tivol@caltech.edu</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> </span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>--</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>For information on how to subscribe/unsubscribe/etc.,
send mail to</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>3dem-request@3dem.ucsd.edu with the text "help"
in the body of the</span></font></p>
<p class=MsoPlainText><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>message.</span></font></p>
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