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<FONT FACE="Verdana"><B>Postdoctoral position in cryo-electron microscopy of vitreous sections</B>.<BR>
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Dear Colleagues<BR>
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The 3dem community is well aware of the advantage of cryo-em of single particles in thin vitrified films. It is a great pity that thick objects such as cells and tissues can’t be observed in the same way. <BR>
Can’t they? <BR>
Yes they can with Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS). <BR>
The great news is that CEMOVIS is now ready for practical use. It starts to show its extraordinary capability.<BR>
The trouble is that CEMOVIS is a demanding technique. It requires that comparatively large volumes of biological material are vitrified and cut into thin vitreous sections. It requires skill, experience and obstinacy.<BR>
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We have been working on CEMOVIS since 20 years and we feel the method is ripe now. CEMOVIS makes it possible to preserve cells and tissues in native state down to atomic resolution. What CEMOVIS shows is surprisingly different from what is known from previous studies and is shown with much finer details. The limitation is no more set by the preservation of the specimen, but by our capability of retrieving the overabundant information in the image. Computer tomography will be essential for further development. We are working in close collaboration with Baumeister’s group in Munich (</FONT><FONT FACE="Times New Roman">http://www.biochem.mpg.de/baumeister/).<BR>
</FONT><FONT FACE="Verdana">Good results have been slow to come, but they are now flowing in a rapidly growing stream. Please consult the following references.<BR>
<FONT SIZE="2">Dubochet, J., and N. Sartori Blanc. 2001. The cell in absence of aggregation artifacts. <I>Micron</I>, 32:91-99.<BR>
Leforestier, A., J. Dubochet, and F. Livolant. 2001. Bilayers of nucleosome core particles. <I>Biophys. J.</I>, 81:2414 - 2421.<BR>
Sartori Blanc, N., A. Senn, A. Leforestier, F. Livolant, and J. Dubochet. 2001. DNA in Human and Stallion Spermatozoa Forms Local Hexagonal Packing with Twist and Many Defects. <I>Journal of Structural Biology</I>, 134:76-81. <I>Selected as “Article of the year” by the Journal of Structural Biology<BR>
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<B>We are looking for a post-doctoral scientist experienced with cryo-electron microscopy in order to reinforce our group in this exciting time. The position is available now.<BR>
A position for a PhD student is also available.<BR>
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Please consult our web site for more information:<BR>
</FONT><FONT FACE="Times">http://www.unil.ch/lau/job.html<BR>
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Prof. Jacques DUBOCHET<BR>
Laboratoire d'Analyse Ultrastructurale<BR>
Bâtiment de Biologe<BR>
Université de Lausanne<BR>
CH-1015 Lausanne, Switzerland<BR>
+21 692 42 80 / Fax: +21 692 41 05<BR>
Jacques.Dubochet@lau.unil.ch<BR>
http://www.unil.ch/lau/<BR>
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