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At 10:53 AM 1/29/2003 +0100, you wrote:<br>
<blockquote type=cite class=cite cite><font face="arial" size=2>I'm
looking for a cheap, fast and reliable supplier (for Europe)
of</font><br>
<font face="arial" size=2>good holey carbon coated grids for
Cryo-TEM.</font><br>
<br>
<font face="arial" size=2>Any suggestions?</font><br>
<br>
<font face="arial" size=2>Thanks everybody,</font><br>
<br>
<font face="arial" size=2>Philip</font><br>
<br>
<font face="arial" size=2>Philip Koeck<br>
Södertörns Högskola and<br>
Karolinska Institutet<br>
Dept. of Bioscience at Novum<br>
S-14157 Huddinge<br>
Sweden<br>
phone: +46-8-6089186<br>
fax: +46-8-6089290<br>
<a href="http://www.biosci.ki.se/em">http</a>://www.biosci.ki<a href="http://www.biosci.ki.se/em">.se/em</a></font><br>
</blockquote><br>
We now routinely use Quantifoil grids for our cryo-EM applications.
<br>
<br>
Other than for special situations do we still produce our own holey or
lacy films due to the convenience of these ready made films. Although not
particularly cheap they are comparable to other suppliers and you save on
the time and effort required if you produce your own.<br>
<br>
We find the Quantifoil films are generally produced reliably and
reproducibly (there has been a vast improvement on the early grids) and
the beauty of them is the defined geometry of the holes which can be
customized to your needs. This can also save you time on the microscope
as searching the grid becomes easier and quicker due to the regular
pattern of the holes.<br>
<br>
Even though our lab is situated in Germany it can take from 2 weeks
to a month for delivery of new batches of grids so you have to make sure
you order them ahead of time.<br>
<br>
regards<br>
<br>
Ken<br>
<br>
<br>
<x-sigsep><p></x-sigsep>
*********************************************************<br>
Ken Goldie<br>
European Molecular Biology Laboratories<br>
Meyerhofstrasse 1<br>
69117 Heidelberg<br>
GERMANY<br>
<br>
Tel: 0049 6221 387 362 (Office), Fax 0049 6221 387
306<br>
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