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<H1 style="TEXT-ALIGN: center" align=center>The Biophysical
Discussions</H1><SPAN
style="FONT-SIZE: 14pt; COLOR: blue; FONT-FAMILY: 'Arial Black'; mso-bidi-font-size: 12.0pt">
<H2
align=center>.......................................................................................</H2>
<P class=MsoNormal style="TEXT-ALIGN: center" align=center>Frontiers in
Structural Cell Biology:</SPAN></P>
<P class=MsoNormal style="TEXT-ALIGN: center" align=center><SPAN
style="FONT-SIZE: 14pt; COLOR: blue; FONT-FAMILY: 'Arial Black'; mso-bidi-font-size: 12.0pt">Determining
the structures of large subcellular machines<?xml:namespace prefix = o ns =
"urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P>
<H2 align=center><FONT color=#0000ff size=6>
<H2 align=center>
<H2 align=center>
<H2 align=center>
<H2 align=center><FONT
size=6>...................................................................</FONT></H2></H2></H2></FONT>Meeting
Dates: April 20-22, 2002.</H2></H2>
<P class=MsoNormal> <U>Meeting Site</U>: Asilomar, California.<SPAN
style="mso-spacerun: yes"> </SPAN>This is a spectacular site. You
can see it at <A
href="http://www.asilomarcenter.com/">http://www.asilomarcenter.com/</A>.</P>
<P class=MsoNormal
align=center>.......................................................................................</P>
<P>The biophysical discussions are set up so that the discussion time is greater
than the times allotted for presentations, which will be available on the web
prior to the meeting.<SPAN style="mso-spacerun: yes"> </SPAN>The format is
unusual, but highly successful. </P>
<P
align=center>.......................................................................................</P>
<P><U>To apply send a letter of intent to </U><A
href="mailto:discussions@biophysics.org">discussions@biophysics.org</A><U> by
November 15, 2001.</U> Space is limited.</P>
<P
align=center>.......................................................................................</P>
<P class=MsoNormal style="TEXT-ALIGN: center" align=center><B
style="mso-bidi-font-weight: normal"><U>Organizing committee</U>: Axel Brunger
(Stanford University), David DeRosier (Brandeis University), Steve Harrison
(Harvard University), and Eva Nogales (University of California at
Berkeley).</B></P>
<P class=MsoNormal>The Program (Saturday, April 20, 9 am to Monday, April 22, 12
noon)</P>
<P class=MsoNormal>Session I.<SPAN style="mso-spacerun: yes">
</SPAN>The state of structural biology of large structures.</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Moderator Helen Saibil</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN><SPAN style="mso-spacerun: yes"> </SPAN>Richard Henderson</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN>The power of electron cryomicroscopy.</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN><SPAN style="mso-spacerun: yes"> </SPAN>Joachim Frank<SPAN
style="mso-bidi-font-size: 10.0pt"><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="mso-bidi-font-size: 10.0pt"><SPAN
style="mso-tab-count: 2">
</SPAN>The Ribosome -- a molecular machine in motion</SPAN></P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN><SPAN style="mso-spacerun: yes"> </SPAN>Jamie Cate</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN><SPAN style="mso-bidi-font-size: 10.0pt">Biochemical basis for x-ray
crystallography of the ribosome</SPAN></P>
<P class=MsoNormal> Session II.<SPAN style="mso-spacerun: yes">
</SPAN>Extending x-ray crystallography to ever larger structures</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Moderator Keith Hodgson</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN><SPAN style="mso-spacerun: yes"> </SPAN>Andy Thompson</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN>Can we routinely collect useful data from micro-crystals?</P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><SPAN
style="mso-spacerun: yes"> </SPAN>Janos Hajdu</P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in"><SPAN
style="mso-tab-count: 1">
</SPAN>Future X-ray sources (tentative)</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN><SPAN style="mso-spacerun: yes"> </SPAN>Randy Reed</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN><SPAN style="mso-bidi-font-size: 10.0pt">The phase problem: does size
matter?</SPAN></P>
<P class=MsoNormal> Session III.<SPAN
style="mso-spacerun: yes"> </SPAN>New ways to obtain large complexes
for structural studies</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Moderator Axel Brunger</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Don Wiley</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN><SPAN style="COLOR: black">Stabilizing multi-component biological
complexes for structural studies by protein engineering, expression, and
refolding - AND - avoiding artifacts<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="COLOR: black"><SPAN
style="mso-tab-count: 1">
</SPAN>TBA<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="COLOR: black"><SPAN
style="mso-tab-count: 2">
</SPAN>Expression and co-expression of components (tentative title)</SPAN></P>
<P class=MsoNormal> Session IV.<SPAN style="mso-spacerun: yes">
</SPAN>What does the future hold for electron cryomicroscopy?</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Moderator Bob Glaeser</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Niko Grigorieff<SPAN style="mso-tab-count: 1"> </SPAN></P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in; TEXT-INDENT: 0.5in">Single
particles always fit the mold</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Ken Downing<SPAN
style="mso-tab-count: 2">
</SPAN></P>
<P class=MsoNormal style="MARGIN-LEFT: 0.5in; TEXT-INDENT: 0.5in">The hybrid
approach to electron crystallography</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Wolfgang Baumeister</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN><SPAN style="mso-bidi-font-size: 10.0pt">Electron Tomography: Towards
visualizing macromolecular assemblies inside cells<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="mso-bidi-font-size: 10.0pt"><SPAN
style="mso-tab-count: 1">
</SPAN>Ed Egelman<o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN-LEFT: 1in"><SPAN
style="mso-bidi-font-size: 10.0pt">Polymorphism, can we detect it? Can we use
it? Can we control it?<SPAN style="mso-spacerun: yes"> </SPAN>Examples
from actin and nucleoprotein complexes.</SPAN></P>
<P class=MsoNormal> Session V.<SPAN style="mso-spacerun: yes">
</SPAN>Can hybrid methods provide credible atomic models?</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Moderator Eva Nogales</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Niels Volkmann</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 2">
</SPAN>Atomic model of the cell: docking in a tomographic environment</P>
<P class=MsoNormal><SPAN
style="mso-tab-count: 1">
</SPAN>Willy Wriggers</P>
<P class=MsoNormal style="MARGIN-LEFT: 1in"><SPAN
style="mso-bidi-font-size: 10.0pt">Reconciling Shape with Structure:
Morphometric Strategies for<BR>Multi-Resolution
Flexing</SPAN></P></DIV></FONT></DIV></BODY></HTML>