[3dem] Very Vague Question

[Ayil Perrott]

Hi, Paul. I hope all is well with you!

In conjunction with what Steven has so eloquently said, it will really
depend on the needs of your collaborators and your available resources. How
it worked for me in my previous experience was that we worked with
collaborators directly to gradually show them the ropes of the processing
software and what to look out for while working with the data, with the
goal of having them conduct processing on their own and come to us with
questions if they were confused. I helped teach numerous collaborators and
new hires about processing, and worked with senior leadership in the core
to help them better understand and be more creative with their pipelines.

Translating to your facility, one of the big questions revolves around how
involved you and your core members want to be with your collaborators in
this respect, and how involved they want to be in the learning process. Are
they able and open to learn how to do image processing, and eventually do
it on their own? If you have the people that are well-experienced with
processing and are willing to teach/directly assist with it, that could
potentially be a great way to help your collaborators with their projects
while also building stronger connections with the core, though of course
there is the problem of proper compensation. My core allowed for its
members to become more involved with collaborators and get on some papers,
though deep involvement with multiple projects personally did not work for
me as a core member.

There are also a slew of amazing resources to provide to collaborators if
they are not entirely familiar with the concepts of cryo-EM that are
critical for processing. One of these is cryoem101.org, a website created
to teach the concepts of cryo-EM and cryo-ET from sample prep to image
processing, all of which I feel is critical for understanding how to work
with the data.

I hope this helps!

Sincerely,
Ayil Perrott
(they/them)
--
Doctoral Student
Physiology and Biophysics
Virginia Commonwealth University
Richmond, VA


On Tue, Aug 12, 2025 at 9:45 AM Chipman,Paul via 3dem <3dem at ncmir.ucsd.edu>
wrote:

> Hi All,
>
>
>
> We recently acquired a TFS Glacios and have started to pump out large
> amounts of data.  Many of our customers are completely unfamiliar with
> image processing.  I’m interested to hear how any other core facilities or
> similar have dealt with this issue.  Since we are an expected revenue
> neutral core I’m a bit challenged on how to best help our users.  Should we
> hire someone to deal with processing? What would be an expected salary?
> Post doc?  We have a bioinformatics core that might be helpful as well or
> positioned to have a joint appointment.  Just looking for thoughts.
>
>
>
> Best,
>
> Paul
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>
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