[3dem] Grid Suggestions Request

[Daniel Asarnow]

Ted Pella sells gold 200 mesh grids with a thicker continuous carbon layer
(on formvar) - I find they are quite robust for a similar purpose. Product
#01808G-F.

https://urldefense.com/v3/__https://www.tedpella.com/Support_Films_html/Support_Films_and_Substrates_Overview.aspx__;!!Mih3wA!DsgT9qYhFM0Qe27VBvdCXPsdjYFcdqGBJmMhr1-IRyL7oXyprPJpcauc6epWirYjSniAjUVu6YLPmZRsFjz5bQs$ 

Best,
-da

On Wed, Oct 9, 2024 at 1:58 PM Watt, John Daniel <watt at lanl.gov> wrote:

> Hello All, We have a cryo-CLEM/FIB workflow with the endpoint being a side
> entry TEM holder. Normal gold grids are too flimsy for our workflow so I
> have been buying Au-Flat grids (https: //www. emsdiasum.
> com/au-flat-for-high-resolution-imaging)
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> Hello All,
>
>
>
> We have a cryo-CLEM/FIB workflow with the endpoint being a side entry TEM
> holder. Normal gold grids are too flimsy for our workflow so I have been
> buying Au-Flat grids (
> https://urldefense.com/v3/__https://www.emsdiasum.com/au-flat-for-high-resolution-imaging__;!!Mih3wA!DsgT9qYhFM0Qe27VBvdCXPsdjYFcdqGBJmMhr1-IRyL7oXyprPJpcauc6epWirYjSniAjUVu6YLPmZRs22J9HZ4$ 
> <https://urldefense.com/v3/__https://www.emsdiasum.com/au-flat-for-high-resolution-imaging__;!!Mih3wA!EtDB_677oJ82-1BipBE1B0pt5JMtHL6wM4_DsPPJoQLHtg1ndaDI4zFjXSRPGmMN6-3JA5NdS7-7JAdb$>)
> as they are more rigid and easy to handle.
>
>
>
> As stated on the webpage;
>
>
>
> *Stronger Au-flat is produced with gold mesh grids that are about 6
> microns thicker than typical Cryo-EM grids. This makes the grids stiffer
> and less likely to bend or deform. The increased thickness is fully
> compatible with side-entry holders as well as auto-loaders.*
>
>
>
> However, the AuPd allow film itself is incredibly fragile and we find
> around 50% of the grids arrive unusable. Also the film often degrades
> during our workflow, which reduces the cell count on grid. We don’t
> necessarily need the AuPd film and would be ok with normal C film.
>
>
>
> The question is – *does anyone know of grid manufacturers that offer the
> thicker, more rigid, gold mesh grid but with a standard C film?*
>
>
>
> Many thanks, John.
>
>
>
>
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