[3dem] GP2 Humidity

[denis.chretien at univ-rennes1.fr]

We use the same trick as Oleksiy with our EMGP. It is only necessary to turn it on a few seconds before loading a grid since it reaches very quickly 99% humidity (kept at the minimum), and we turn it off before plunging to avoid possible contamination of ethane with water. With this model, you can even choose the colour of the light inside the humidifier ;-) (or let it alternate).

best,

Denis


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> Le 24 sept. 2024 à 19:29, Kovtun, Oleksiy <oleksiy.kovtun at mpinat.mpg.de> a écrit :
> 
> Hi Len, 
> 
> it may be airflow  problem. Check that and side chamber ( top of back wall) and outside (on the top) do spin.
> 
> You can also use a en external humidifier with long silicon tube to direct water mystery into the chamber via on of the sample loading ports. We use Honeywell HH210E <https://urldefense.com/v3/__https://www.kika.at/honeywell-kaltluftbefeuchter-hh210e-23923396.html__;!!Mih3wA!AAbnLuJxYznYEczzGG0BVb5eKUK_LDmUP573exVJubfnNFWy8Xi6W0BUL8SZZc98VeQrJjF4DGr6oYJtvLHAaPMvyNvlJbN1oA$> as it has a convent opening where we can place 3D printed adapter to attached the tube. 
> 
> No humidity control, but we want humidity close to saturation anyway. Sticking the tube in for sample loading and removing it before the plunging works well. 
> 
> Best, 
> 
> Oleksiy 
> 
> 
> Dr Oleksiy Kovtun
> ------------------------------
> Max-Planck Research Group Leader
> MPI for Multidisciplinary Sciences Göttingen, Germany
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> 
> 
> 
>> On 24. Sep 2024, at 19:04, Thomas, Leonard M. <lmthomas at ou.edu> wrote:
>> 
>> Hello All,
>>  
>> We are having a problem with our Leica GP2 cryo plunger.  It is failing to reach or maintain the set humidity.  Any ideas as to what to look at would be greatly appreciated before having Leica come out.
>>  
>> Thanks You,
>> Len Thomas
>>  
>> Leonard M. Thomas, Ph.D.
>> Biomolecular Structure Core Director
>> Oklahoma COBRE in Structure Biology
>> Department of Chemistry and Biochemistry
>> Stephenson Life Science Research Center
>> 101 Stephenson Parkway
>> Norman, OK 73019
>> (405)325-1126
>> https://urldefense.com/v3/__https://www.ou.edu/structuralbiology/cobre-core-facilities/mcl__;!!Mih3wA!C7-DyGinyYgzw25BsAMh8OEWncT8bAdSY7pF-70_RkTaSZ57YYm88vDE8a7j0DYsBA2hhrgrYiEBufkTXkQKYSd4lz7h-JvSaJU$  <https://urldefense.com/v3/__https://www.ou.edu/structuralbiology/cobre-core-facilities/mcl__;!!Mih3wA!GdwUkvw5yVL4PEev5cW7UMRm6yNjJLcunTqYnSd1wfQsbP6erhfPscc9TpPnqE1rd2yIL0gEqiKae3wQnxw$>
>>  
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