[3dem] Post-processing of dataset from carbon-coated grids: noise pb

[Takanori Nakane]

Hi,

 > we have a question concerning post-processing of pctls from
 > carbon-coated grids.
 >
 > We see a lot of noise in our map.

We need more information!

What do you mean by "a lot of noise"?
Which software did you use?
Was the map bad from the beginning, or did it become
bad after a certain step of processing?

Does the FSC curve look reasonable?
Isn't the map over-sharpened?
If there are huge variations in the local resolution,
one number might not be adequate.

In general, there is nothing special about carbon-coated grids.

Best regards,

Takanori Nakane

On 3/15/24 01:17, Francesca GUBELLINI wrote:
> 
> Dear EM community,
> 
> 
> we have a question concerning post-processing of pctls from 
> carbon-coated grids.
> 
> We see a lot of noise in our map.
> 
> 
> Anyone has specific tips on how to process this kind of data?
> 
> 
> Thanks a lot!
> 
> 
> Francesca and Sophia
> 
> 
> 
>   ---------------------------------
> Francesca Gubellini, PhD
> Institut Pasteur
> Unité de Microbiologie Structurale
> Bat. Metchnikoff
> 25 rue du Dr Roux
> 75015 Paris
> France
> Tel: +33 1 40 61 39 84
> ----------------------------------
> 
> 
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