[3dem] glow discharge carbon coated grids several times?

[Daniel Asarnow]

Hi Tobias,
In my experience, it is usually fine, but depending on the amount of
carbon, mesh spacing, and handling technique they will accumulate more
damage over time. For example 400 mesh 2/2 µm is stronger than 200 mesh
3.5/1 µm which are almost "all hole." You can see the foil readily on a
light microscope like used in tissue culture if you want to make sure they
are still good before use.

Best,
-da

On Fri, Sep 8, 2023 at 6:09 AM Tobias Furstenhaupt <furstenh at mpi-cbg.de>
wrote:

> Dear colleagues,
>
> we regulary use holey carbon coated copper grids in our plunge freezer.
> Naturally we glowdischarge them before. We use a Pelco easiglow at 15 mA,
> 0.4 mbar for 30 seconds on each side).
> To be on the safe side, we glow discharge always 1-2 grids more than we
> probably need for plungefreezing. Does anybody of you have any experience
> if leftover grids can be reused? Ie that we put the grids back in the box
> and glow discharge them a second time and use them?
> Formvar coated grids dont survive a second glow discharge (the plastic
> rips broadly open) but what about a carbon coat? Does anybody know?
>
> cheers
> Tobias
>
>
> -----------------------------------
> Tobias Fürstenhaupt, PhD
> head of Electron Microscopy
> Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
> Pfotenhauerstrasse 108
> 01307 Dresden, Germany
>
> mail: furstenh at mpi-cbg.de
> phone: (+49) (0)351 210-2690
> cell: (+49) (0)176 / 44498706 (NEW!)
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