[3dem] best practice question for ThermoFisher microscopes

[Parvate, Amar D]

Hey David
In my experience, the Alignment files are one level higher than the FEG registers (Alignment files > FEG registers). The FEG registers store settings based on your alignment files. Other way round, you could always update your FEG registers if you have a new/better alignment or overwrite an older FEG register. But a FEG register wont correct a misalignments.
Would also love the hear from the EM community what their experience has been.
Best
Amar

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Morgan, David Gene
Sent: Thursday, May 4, 2023 1:22 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] best practice question for ThermoFisher microscopes

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Hi,

I recently realized that different TFS service engineers have told us different things about best practices for using the FEG registers and alignment files on our Talos Arctica.  One of the engineers told us to use the FEG registers to store the current best FEG settings and to use a series of alignment files to store things like commonly used imaging conditions for different tasks.  Another engineer said to use FEG registers for both purposes.

Is there a general consensus on this, and if not, what do people think are the advantages of using one over the other?

Thanks in advance for any responses.

--
    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047E Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
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