[3dem] 3dem Digest, Vol 190, Issue 1

Thu Jun 1 06:27:16 PDT 2023

AMAZING! 

I had just started a new email to the group, because Linde had come back to us with a pretty ridiculous offer in order to continue their supply. They offered to keep supplying the mixture under the proviso that every order contains at least 6x 10l bottles and they doubled the price to €900 (I assumed pre tax). They were willing to extend the current 12 months warranty to 36months, but nevertheless, we use max one bottle per year, so would still be pretty much wasting 2-3 bottles at the end of an order cycle (and that is assuming Linde won't scrap the equipment in the meantime due to absolutely no orders in the next 6 years with that hare-brained idea). 

So these news are fantastic! Thanks for tracking them down! 

Best wishes, 

Cornelia 

From: 3dem-request at ncmir.ucsd.edu 
To: "3dem" <3dem at ncmir.ucsd.edu> 
Sent: Thursday, 1 June, 2023 15:03:48 
Subject: 3dem Digest, Vol 190, Issue 1 

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Today's Topics: 

1. Re: Calling all groups who use cryogen mix from Linde-Gas 
(Germany, maybe Austria, Switzerland?) for plunging (Dario) 
2. Postdoc position - computational tools for ?in situ? cryo-EM 
(Briggs, John) 
3. Group Leader postion in cryo EM / cryo ET @ Institut Pasteur, 
Paris (Michael NILGES) 

---------------------------------------------------------------------- 

Message: 1 
Date: Thu, 1 Jun 2023 10:05:48 +0200 
From: Dario <dariobrack at googlemail.com> 
To: 3dem at ncmir.ucsd.edu 
Subject: Re: [3dem] Calling all groups who use cryogen mix from 
Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging 
Message-ID: 
<CAHOoyBOLpbBBUf9YABm0sM5WQR9aaY9QbtN_jr43J-VBWPqPqw at mail.gmail.com> 
Content-Type: text/plain; charset="utf-8" 

Just for everyones information: 
After contacting basically each gas supplier in Germany, I finally found 
one that can produce and deliver the liquid Ethane:propane mixture (at 
least in Germany) 

Nippon-gases 
https://urldefense.com/v3/__https://nippongases.com/__;!!Mih3wA!EWcu8XZh3yPCwu6EsK7-hEtzSFtvLJcLuVmsBcMPfQi6BJeV1_kJpKhkAHqLrcZJSuWkePAPJ4sLNBH6RLdij0fTGA$ 

Cornelia Cazey <cornelia.cazey at cssb-hamburg.de> schrieb am Di., 25. Apr. 
2023, 13:50: 

Hi Oliver, 

Thanks a lot for the info. But why do they only supply in 50l cylinders? 
Or rather why order a 50l cylinder? Economic reasons? 

Thanks! 

Cornelia 
On 25 Apr 2023, at 13:40, Oliver Hofnagel < 
oliver.hofnagel at mpi-dortmund.mpg.de> wrote: 

BQ_BEGIN

Dear Cornelia, 

here our supplier for 37% ethane / 63% propane 50l cylinder cryogen mix 
in Germany: 

*AIR LIQUIDE Deutschland GmbH Kunden-Service-Center Spezialgase 
Bataverstra?e 47 47809 Krefeld Telefon: (02151) 954-254 **Email : 
spezialgase.ald at airliquide.com <spezialgase.ald at airliquide.com>* 

Its important to order a 50l cylinder, not a 10l cylinder ! 

Greetings, 

Oliver 

Dr. Oliver Hofnagel 
Max-Planck-Institute of Molecular Physiology 
Electron Microscopy 
Otto-Hahn-Str. 11 
44227 Dortmund 
Germany 

------------------------------ 
*Von:* 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von Michael Elbaum < 
michael.elbaum at weizmann.ac.il> 
*Gesendet:* Donnerstag, 20. April 2023 16:29:00 
*An:* r.i.koning at lumc.nl; cornelia.cazey at cssb-hamburg.de; 
3dem at ncmir.ucsd.edu 
*Betreff:* Re: [3dem] Calling all groups who use cryogen mix from 
Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging 

Hi. would it be possible to use two pure tanks and a mixing regulator? I 
think such regulators are used for welding. 

Michael 
------------------------------ 
*From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of 
r.i.koning at lumc.nl <r.i.koning at lumc.nl> 
*Sent:* Thursday, April 20, 2023 5:15:10 PM 
*To:* cornelia.cazey at cssb-hamburg.de; 3dem at ncmir.ucsd.edu 
*Subject:* Re: [3dem] Calling all groups who use cryogen mix from 
Linde-Gas (Germany, maybe Austria, Switzerland?) for plunging 

Dear Cornelia, 

Our most recent order of ethane/propane also did not come through from 
Line because of the same reason. We will have to do with ethane only for 
now but I prefer the mixture for obvious reasons, though we do manage with 
ethane only. We did not look for nor found another vendor. 

Best regards, 

Roman 

*Dr. R.I.Koning* 

Leiden University Medical Center, Cell and Chemical Biology, Electron 
Microscopy 

Postal Zone S1-P, P.O.Box 9600, 2300 RC, Leiden, The Netherlands 

*visiting address.* Einthovenweg 20, Building 2, zone S1-P, room R-90-21 

*tel.* (31) 71 526 9296 *fax.* (+31) 71 526 8270 *mail.* 
r.i.koning at lumc.nl *web. *Electron Microscopy Facility 
<https://urldefense.com/v3/__https://ccb.lumc.nl/electron-microscopy-198__;!!Mih3wA!GxgfkasxXThNdN_k1a9iqn0d9uls-PLaHnrrJAIhFXNITn6ehlzCx_U6V-rQFPvgm8pSGK1CJ1Vd55FxuUK3uvc$> 

*From:* 3dem <3dem-bounces at ncmir.ucsd.edu> *On Behalf Of *Cazey, Cornelia 
*Sent:* donderdag 20 april 2023 15:52 
*To:* 3dem at ncmir.ucsd.edu 
*Subject:* [3dem] Calling all groups who use cryogen mix from Linde-Gas 
(Germany, maybe Austria, Switzerland?) for plunging 

Dear list participants, 

Our lab is in Germany, and this is a very particular question relating to 
a German supplier, so apologies for addressing the entire list, but it 
seemed the easiest way to find groups who may be able to help. 

We recently learned that Linde-Gas, our supplier for the cryogen mix we 
use in our facility (37% ethane, rest propane) is no longer able to supply 
this mixture due to the overall difficulties in the production, age and 
poor state of their necessary equipment. 

I spoke with the supplier yesterday and while they would like to continue 
to offer the mix, they feel there is not enough market to make it feasible. 
I am now looking for info from groups who are using a cryogen mix rather 
than pure ethane for plunging (I am not sure, but relatively convinced 
that the process would be the same for any kind of mixture, so I guess the 
exact percentages won't matter too much) and have previously sourced 
this from Linde-Gas, and might be or have already been affected by this 
decision. If you have been affected, did you find a solution, a different 
supplier or any other alternative you are willing to share? Or if you 
didn't yet know, how much would this news affect your ability to work, and 
would you be interested to try and convince Linde-Gas to keep going? I 
understand the production line has already been shut down, but could be 
revived with enough interest, however, it may also come with a price 
increase (what doesn't). 

Any help would be greatly appreciated, whether info on alternatives or 
willingness to push for the supply. I am happy to collect replies to send 
to the Linde-Gas representative in bulk or, of course, for those who prefer 
to contact him directly, do so themselves and increase the pressure to keep 
going/restart production. 

Thanks a lot for your help! 

Best wishes, 

Cornelia Cazey 

Cornelia Cazey (She/Her) 

Cryo-EM Facility 

Centre for Structural Systems Biology (CSSB) 
c/o DESY, Building 15 
Notkestr. 85 
22607 Hamburg 
Germany 

phone: +49-(0)40-8998-87706 
email:*cornelia.cazey at cssb-hamburg.de <cornelia.cazey at cssb-hamburg.de>* 

*"If you don't know it's impossible, it's easier to do"* -Neil Gaiman 

Sometimes I may send emails outside of core working hours. I do not 
expect you to read, or respond outside of your own working hours! 

_______________________________________________ 
3dem mailing list 
3dem at ncmir.ucsd.edu 
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem 

BQ_END

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------------------------------ 

Message: 2 
Date: Thu, 1 Jun 2023 12:13:54 +0000 
From: "Briggs, John" <briggs at biochem.mpg.de> 
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu> 
Subject: [3dem] Postdoc position - computational tools for ?in situ? 
cryo-EM 
Message-ID: <500F7DD0-20CD-49F9-B489-5CB4C18A5B47 at biochem.mpg.de> 
Content-Type: text/plain; charset="utf-8" 

Dear All, 

We have a postdoc position available to develop theoretical frameworks and computational tools for image analysis of ?in situ? cryo-electron microscopy data. You should have a PhD in a relevant subject (computational sciences, physics, biophysics or similar) and a strong interest in the theory and methods of cryo-electron microscopy and image analysis by classical or machine learning approaches. 

In the lab we apply cryo-electron microscopy and image processing to determine the structures and distributions of protein complexes ?in situ? from within viruses, cells and organisms. The Max Planck Institute of Biochemistry has outstanding facilities for cryo-electron tomography, microscopy, and image analysis. The successful candidate will join a large, vibrant community developing and applying these methods. 

Applications by 23rd June via the link on this page: https://urldefense.com/v3/__https://www.mpg.de/20379064/postdoc-position-cell-and-virus-structure__;!!Mih3wA!DkVqxEpeWDpJDwoGB1bUizx9xbiFbQEgV7BhJRhNOSNVEJ91-gDAB4h0TL_wqF26MfkQHnpraoeNu7fBUyUeozc$ 

You can contact me with informal enquiries. 

John Briggs 
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------------------------------ 

Message: 3 
Date: Thu, 1 Jun 2023 13:03:44 +0000 
From: "Michael NILGES" <michael.nilges at pasteur.fr> 
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu> 
Subject: [3dem] Group Leader postion in cryo EM / cryo ET @ Institut 
Pasteur, Paris 
Message-ID: <BF3808F4-498A-44EE-8788-3D9A7C754962 at pasteur.fr> 
Content-Type: text/plain; charset="us-ascii" 

Dear 3DEM community, 

The Structural Biology and Chemistry Department at Institut Pasteur has an opening for a junior / mid career group leader in electron microscopy. 
The deadline for applications is July 15, 2023. 

<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ > 

[cid:3433ad4b-4074-41d4-8515-c550cc1620ab at corp.pasteur.fr] 

<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ > 

Junior/Mid-career Group Leader position in Structural Biology/Cryo-electron microscopy & tomography - Research<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ > 
research.pasteur.fr<https://urldefense.com/v3/__https://research.pasteur.fr/en/call/junior-mid-career-group-leader-position-in-structural-biology-cryo-electron-microscopy-tomography/__;!!Mih3wA!ApBbafdgZUYubegPYjaYSoE5QtF_bGMDjjFfFuZL5ROfksfaE43TOrNtOIjQeVHTXv-UDjTU8syV10OueFiyAGbwJX2grw$ > 

Best regards 

Michael 

=============== 
Prof. Michael Nilges 
Head of Structural Bioinformatics Unit 
Vice president of Technology 
Institut Pasteur, Paris 
Tel: +33 1 45 68 82 30 
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