[3dem] Graphene Oxide grids hydrophobic

[Erik Župa]

Hi Dario,

for any graphene oxide grids that are stored longer after preparation you
have to apply plasma cleaning but very short, maybe even 1-3 s to make sure
that graphene oxide won't be destroyed. Graphene oxide grids lose their
hydrophilicity over time so you need to reactivate it.
If you are not sure about the presence of a graphene oxide layer, you can
verify it by loading one grid at room temperature to a microscope and check
the diffraction pattern. There are several papers describing it.


Erik Zupa

Postdoctoral researcher

Jimah lab

Molecular Biology Department

Princeton University



On Thu, Dec 7, 2023 at 11:48 AM Dario <dariobrack at googlemail.com> wrote:

> Dear all,
>
> I have ordered commercial GO grids (QF 300 Cu R2/2 GO) and expected them
> to be hydrophilic out of the box due to the GO layer.
> However, the grid was not covered by the 3ul sample drop and appeared
> quite hydrophobic. How do you prepare the GO grids for plunging?
>
> I plasma cleaned them with air for 8sec, after that they were very
> hydrophilic, but I am afraid I might have destroyed the GO layer.
>
> Best
>
> Dario
>
>
>
>
> _______________________________
>
> Dr. Dario Saczko-Brack
> Postdoc
>
> Ludwig-Maximilians-Universität München
> Department of Cellular Physiology
> Biomedical Center Munich - BMC
> Großhaderner Str. 9
> D-82152 Planegg-Martinsried
>
> Prof. Dr. Claudia Veigel
>
> Room: N.C 01.033
> Phone: +49 (0) 89 2180 71588
> Fax: +49 (0) 89 2180 71732
> Email: Dario.Saczko-Brack at med.lmu.de
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