[3dem] Amyloid processing

[Sjors Scheres - MRC LMB]

Dear EM-ers,

We are getting quite a few requests for help with helical reconstruction
of amyloids. To help newcomers to this field, we recently wrote this
paper in Faradays Discussions:
https://urldefense.com/v3/__https://pubs.rsc.org/en/content/articlelanding/2022/FD/D2FD00034B__;!!Mih3wA!CPrR3QFSKOkAIeOpp9oIs3mrHc0qQKTt6Ot10GdTSzA04v8aRXv0Ky0aKAmrhya2uSPNGT-mqEyyBWpiDAs8Sfqggl8$ 

It contains a detailed description by Sofia Lovestam on three of her
data sets of in vitro assembled tau filaments. You can find these data
sets, together with the full RELION project directories, on EMPIAR. The
idea is that going over these examples yourself, using Sofia's
processing as a guide, will basically be like an advanced amyloid tutorial.

Have fun!

Sjors


-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
https://urldefense.com/v3/__http://www2.mrc-lmb.cam.ac.uk/groups/scheres__;!!Mih3wA!CPrR3QFSKOkAIeOpp9oIs3mrHc0qQKTt6Ot10GdTSzA04v8aRXv0Ky0aKAmrhya2uSPNGT-mqEyyBWpiDAs8kv3uhQQ$