[3dem] Cryo-electron microscopy of proteins

[Debasish Kumar Ghosh [MAHE-KMC]]

Dear Colleague,

I am reaching out to know for a possibility of a doing cryo-electron microscopy of a couple of proteins to understand their structures. We have a couple of interesting endoplasmic reticulum proteins which are required for proteostasis. Recently, we found an insertion mutation (25 amino acid insertion) in one protein, leading to fatal type osteogenesis imperfecta in an individual. Since the structure of the wild-type protein in not solved, we are curious to understand the structure of the wild-type and the mutant proteins to understand the structural basis of the pathogenicity. Besides having the clinical data, we also have the preliminary data of cellular pathophysiology. Also, we have the bacterially expressible clones, and we have also standardized purifying recombinant wild-type and mutant proteins.

I though one can help us in doing the structural study of the proteins by cryo-electron microscopy. I will be extremely happy to collaborate on this project and beyond.

I am happily open for a further discuss on this in a more extensive manner.

Best regards,
Debasish
_________________________________
Debasish Kumar Ghosh, Ph.D.

Assistant Professor
Department of Medical Genetics
Kasturba Medical College, Manipal
Manipal Academy of Higher Education
Manipal, Karnataka, India

Email: debasish.kumar at manipal.edu, dghosh7 at gmail.com
Tel: +91-8910689391, +91-8202923419

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