[3dem] Cryo-electron Microscopy (cryo-EM) Core Specialist

[Miller, Eric]

Van Andel Institute (VAI)<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.vai.org_vari.aspx&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=Y4ft2dDxN28eBsh2vUJ4HmPkoNHxkkF-rSDqmoRipN3-4lXnqHyiR50WBiy5gUbw&s=dHG8YtigrEHhUt2ibQ1_asIzFgzZjDUsmrQAwSODBRA&e= > is a leading independent biomedical research facility dedicated to determining the epigenetic, genetic, molecular and cellular origins of cancer, Parkinson's and other diseases, and translating those findings into effective therapies. The Institute is home to more than 400 scientists and support staff that work in on-site laboratories and in collaborative partnerships that span the globe.



We are currently seeking to hire a cryo-EM Core Specialist to assist in the daily operations of our state-of-the-art cryo-EM facility<https://urldefense.proofpoint.com/v2/url?u=https-3A__cryoemcore.vai.org_&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=Y4ft2dDxN28eBsh2vUJ4HmPkoNHxkkF-rSDqmoRipN3-4lXnqHyiR50WBiy5gUbw&s=u-eOvo23C9xwf4YLH1NYQJpftRb-wo-V0u0YIYRwyqw&e= >, which includes the latest Titan Krios microscope. The cryo-EM Core provides both transmission electron microscopy (TEM) and cryo-EM sample preparation and data collection services to researchers.



As our cryo-EM Core Specialist, you will work with the cryo-EM Core Manager and will be responsible for the following Key Areas:


*         Prepare sample with fixation, dehydration, staining and embedding for transmission electron microscopy; use an ultramicrotome with a diamond knife for routine thin sectioning.
*         Prepare sample with high-pressure freezing, freeze substitution, use cryo-ultramicrotomy for thin sectioning.
*         Prepare sample for Immuno-electron microscopy.
*         Maintains all supplies at adequate levels according to user program needs.
*         Cleans and organizes laboratory spaces, equipment and supplies.
*         Operate hardware associated with EM data collection such as microscope, electron detector.
*         Record projects and experimental conditions in an electronic laboratory notebook.
*         Communicate experimental details with the users and other relevant project team members.
*         Help users prepare the experimental method section in resulting publications.
*         Other duties as assigned.



What skills and qualifications should you possess?


*         We would expect you to possess a Bachelor's or Master's or Ph.D. Degree (or equivalent) in Biochemistry, Biology, Chemistry, or related field.
*         3 - 5 years of hands-on experience in TEM sample preparation is required and ideally you would be familiar with modern electron microscopes, electron detectors, and EM data collection procedures.
*         Experience in a core or shared resource laboratory





Most importantly, you should be a person with the following traits and characteristics:


*         A good communicator who is flexible and has a customer service-oriented personality. We expect you to have a strong commitment to building and maintaining a positive work environment.
*         A motivated and hardworking individual, who has excellent organizational and time management skills.
*         Demonstrate excellent organizational and multitasking skills and ability to meet deadlines.
*         A strong desire and capability to learn new technologies.
*         The ability to work alone or as a team member.
*         Strong critical thinking and troubleshooting skills.





How to apply:

If you possess these attributes and the desire to help us provide best-in-class service to our researchers, we would welcome speaking with you and strongly encourage you to apply today<https://urldefense.proofpoint.com/v2/url?u=https-3A__jobs.silkroad.com_vai_VAICareerSite_jobs_861&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=Y4ft2dDxN28eBsh2vUJ4HmPkoNHxkkF-rSDqmoRipN3-4lXnqHyiR50WBiy5gUbw&s=7LnoWQOkOm1TkdIyZqOy21A2YfVQVqaBq0EvwHpu14Y&e= >.

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