[3dem] Postdoc and Research Scientist positions in Structural Virology

[Kelly Lee]

Postdoctoral Fellow and Research Scientist positions in structural biology and biophysics of infectious disease

Kelly Lee's Lab, University of Washington, Seattle

Our lab applies cryo-electron tomography, single particle cryo-EM, and biophysical approaches including structural mass spectrometry (H/D exchange-MS) to investigate the machinery and mechanisms of infection by human viruses. We are fascinated by the dynamic aspects of enveloped virus entry and membrane fusion and apply a range of complementary methods to understand the structural and dynamic origins of antigenic and immunogenic variation in rapidly evolving viruses such as HIV, influenza, and other RNA viruses.

To learn more about our work, see for example:

Cryo-ET of Env on Intact HIV Virions Reveals Structural Variation and Positioning on the Gag Lattice <https://urldefense.com/v3/__https://www.cell.com/cell/fulltext/S0092-8674(22)00071-X__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav34UvikpP$  >
Mangala Prasad V, Leaman D, Lovendahl K, Croft JT, Hodge EA, Benhaim MA, Zwick MB, Lee KK
Cell. 2022 Feb 17 185:1-13
 
Structural Dynamics Reveal Isolate-specific Differences at Neutralization Epitopes on HIV Env <https://urldefense.com/v3/__https://www.cell.com/iscience/pdf/S2589-0042(22)00720-9.pdf__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav36KulWWh$  >
Hodge EA, Naika GS, Kephart SM, Nguyen A, Zhu R, Benhaim MA, Guo W, Moore JP, Hu S-L, Sanders RW, Lee KK
iScience. 2022 Jun 17 25:104449

Structural Monitoring of a Transient Intermediate in the Hemagglutinin Fusion Machinery on Influenza Virions <https://urldefense.com/v3/__https://www.science.org/doi/10.1126/sciadv.aaz8822__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav3_ZwAigV$  >
Benhaim MA, Mangala Prasad V, Garcia NK, Guttman M, Lee KK
Science Advances 2020 Apr 29;6(18):eaaz8822
 
Visualization and Sequencing of Membrane Remodeling Leading to Influenza Virus Fusion <https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/27226364/__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav3zt71Y6U$  >
Gui L, Ebner JL, Mileant A, Williams JA, Lee KK
Journal of Virology 2016 Jul 11;90(15):6948-6962

For more information contact Kelly Lee at kklee at uw.edu <mailto:kklee at uw.edu> (https://urldefense.com/v3/__https://www.lee-lab-fusion.org__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav32YX1_XW$   <https://urldefense.com/v3/__https://www.lee-lab-fusion.org/__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav38AiTkC8$  >) 

Publications: https://urldefense.com/v3/__https://www.lee-lab-fusion.org/publications__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav3_XnC7tY$  

Responsibilities

Develop and apply cryo-electron tomography approaches, single particle cryo-EM, or complementary quantitative biophysical methods such as mass spectrometry, fluorescence microscopy and computational modeling to analyze protein-mediated membrane fusion machinery and interactions with host factors such as receptors, membranes, and neutralizing antibodies. Opportunities for training in additional techniques and collaborations in virology and immunology are available.

Qualifications

The successful candidate must have a Ph.D. or equivalent degree. Experience in cryo-EM/ET with single-particle or subtomogram averaging is highly desirable, though candidates with expertise in biophysics, structural biology, or computational modeling methods are also encouraged to apply.

________________________________

Kelly Lee, Ph.D.

Professor
Department of Medicinal Chemistry
University of Washington

Co-director
Biological Physics Structure and Design Graduate Program
University of Washington

kklee at uw.edu
https://urldefense.com/v3/__http://faculty.washington.edu/kklee__;!!Mih3wA!Ajyp_7dB6PWQsXiWGUPrfERYkDqvE0llXjBs4lK4YxJSkC6Vg4vquU6G-kJUOIsR8KtzEhav36lYFJfZ$  

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