[3dem] [EXTERNAL] Re: Problem getting top off pin type autogrid grid boxes

Fri Sep 24 07:19:38 PDT 2021

Hi Bob,

Developing better tools for handling grid boxes is an area that we have
been looking at recently. As mentioned, many labs are using the "off-label"
mechanical/drafting pencil approach. These pencils are far from optimal for
gripping or for use in LN2.

We would welcome input from the community on what they might like to see in
an optimized gripper tool or other tools in the sample prep workflow.

Best wishes,
Ben
--
Benjamin Apker
Chief Executive Officer
MiTeGen

See our Cryo-EM Lab Tool Kits
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On Fri, Sep 24, 2021 at 10:00 AM Grassucci, Robert <rg2502 at cumc.columbia.edu>
wrote:

> Hi Michael
> I have tried that and the tip comes loose again.  I think I will try crazy
> glue or epoxy on it as suggested by Bill Rice.  What would be a better
> design would be reversing the threads so loosening the top would tighten
> the tip and tightening the top would loosen it.  That way you could not get
> tighten it in the first place.
> Bob
>
> Sent from my iPhone
>
> On Sep 24, 2021, at 9:53 AM, Michael Elbaum <michael.elbaum at weizmann.ac.il>
> wrote:
>
> 
>
> Hi Bob,
>
>
> Applying a bit more downward force than you'd first feel comfortable with
> seems to tighten up the threads in the tool so they don't slip. I've never
> broken the pin or anything else, but it's not inconceivable. Just push
> straight down. You can also swap out the official tool for a mechanical
> pencil (Staedler Mars technico 788), with which it's hard to overtighten in
> the first place.
>
>
> best,
> Michael
> ------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Walsh, Richard
> Michael <Richard_Walsh at hms.harvard.edu>
> *Sent:* Friday, September 24, 2021 4:40:02 PM
> *To:* Grassucci, Robert; 3DEM List
> *Subject:* Re: [3dem] Problem getting top off pin type autogrid grid boxes
>
> Hi Bob,
> we use a different autogrid box tool for overtightened boxes. These tend
> to frost up pretty quickly but since there is no screw it does not come
> apart. We have this one:
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_grids-5Fhtml_gridbox.htm-23-5F160-2D46&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=MXmUAqVH7G8sgLS25UU5Jlom8rG0D___PLNtnh1XUa8&s=OWpnxlX9O_5XWHlCtDlYOtwfb4ht1IlTx7RsNcfRv6E&e= 
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_grids-5Fhtml_gridbox.htm-23-5F160-2D46&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=9Vz0DVD6xGW1W6nXr26jJVJ5gugMKgcnvylKDSIgxfg&e=> but
> it seems mitogen also makes something similar:
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.mitegen.com_product_gripper-2Dtool-2Dfor-2Dmetal-2Dgrid-2Dboxes_&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=MXmUAqVH7G8sgLS25UU5Jlom8rG0D___PLNtnh1XUa8&s=DUl4xBYWIvMiry4HaGLY5hdoeEfLOcSbNuxehycQEHM&e= 
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.mitegen.com_product_gripper-2Dtool-2Dfor-2Dmetal-2Dgrid-2Dboxes_&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=FImsT2YWrDoUFeZTzBQjJmYVUW83yqCGO6ImVbaBlMc&e=>
> Gripper Tool for Grid Boxes | MiTeGen
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.mitegen.com_product_gripper-2Dtool-2Dfor-2Dmetal-2Dgrid-2Dboxes_&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=FImsT2YWrDoUFeZTzBQjJmYVUW83yqCGO6ImVbaBlMc&e=>
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> <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mitegen.com&d=DwQF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=UnXiI86jUyBaYaIJaWvWNn_J7A27whVlq5swN45pW4w&e=>
>
> best regards,
>
> Richard
>
> Richard M. Walsh , Ph.D.
>
> The Harvard Cryo-EM
> Center for Structural Biology
>
> Harvard Medical School
> 250 Longwood Avenue
> SGM 102
> Boston MA 02115
>
> Phone: (617) 432-9042
> Email:Richard_Walsh at hms.harvard.edu <Richard_Walsh at hms.harvard.edu>
> Web: cryoem.hms.harvard.edu
> <https://urldefense.proofpoint.com/v2/url?u=http-3A__cryoem.hms.harvard.edu_&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=Rj45yjtnLOrsylhyd8CXkFT0xrNiYEKRWSKBY6zPtnM&e=>
> ------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Grassucci, Robert
> <rg2502 at cumc.columbia.edu>
> *Sent:* Friday, September 24, 2021 9:34 AM
> *To:* 3DEM List <3dem at ncmir.ucsd.edu>
> *Subject:* [3dem] Problem getting top off pin type autogrid grid boxes
>
> Hi all
> We have been having a problem where users tighten the tops of the autogrid
> grid boxes and the tool’s metal tip unscrews before the top does.  Has
> anyone found some way to better secure the metal tip of the grid box top
> tool so it won’t spin or ensured the grid box top would loosen before the
> tip of the tool?  Thank you.
> Bob
>
> Sent from my iPhone
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