[3dem] Problem getting top off pin type autogrid grid boxes

[Michael Elbaum]

Hi Bob,


Applying a bit more downward force than you'd first feel comfortable with seems to tighten up the threads in the tool so they don't slip. I've never broken the pin or anything else, but it's not inconceivable. Just push straight down. You can also swap out the official tool for a mechanical pencil (Staedler Mars technico 788), with which it's hard to overtighten in the first place.


best,
Michael

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Walsh, Richard Michael <Richard_Walsh at hms.harvard.edu>
Sent: Friday, September 24, 2021 4:40:02 PM
To: Grassucci, Robert; 3DEM List
Subject: Re: [3dem] Problem getting top off pin type autogrid grid boxes

Hi Bob,
we use a different autogrid box tool for overtightened boxes. These tend to frost up pretty quickly but since there is no screw it does not come apart. We have this one: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_grids-5Fhtml_gridbox.htm-23-5F160-2D46&d=DwIF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=A-U8hHexbpineZj5CPCin1wZ7eI3pGwhke9dRx44eCw&s=wx31N4OEhwU5E6ANU_1KPkear7FksDsFCs7GwDVPmck&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_grids-5Fhtml_gridbox.htm-23-5F160-2D46&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=9Vz0DVD6xGW1W6nXr26jJVJ5gugMKgcnvylKDSIgxfg&e=> but it seems mitogen also makes something similar: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.mitegen.com_product_gripper-2Dtool-2Dfor-2Dmetal-2Dgrid-2Dboxes_&d=DwIF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=A-U8hHexbpineZj5CPCin1wZ7eI3pGwhke9dRx44eCw&s=8vSQWH-ePgu_MCSyNmtnagikd_MekBcSbC-8CdhW4DA&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.mitegen.com_product_gripper-2Dtool-2Dfor-2Dmetal-2Dgrid-2Dboxes_&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=FImsT2YWrDoUFeZTzBQjJmYVUW83yqCGO6ImVbaBlMc&e=>
Gripper Tool for Grid Boxes | MiTeGen<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.mitegen.com_product_gripper-2Dtool-2Dfor-2Dmetal-2Dgrid-2Dboxes_&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=FImsT2YWrDoUFeZTzBQjJmYVUW83yqCGO6ImVbaBlMc&e=>
Menu. Products – . Crystallography – Everything you need for crystallization, crystal harvesting, cryocooling, and data collection ; Cryo-EM – Tools and accessories for sample handling, cryocooling and cryostorage; Lab Equipment & Instruments – Incubators, stereo microscopes, dry shippers, refrigerators and other essential lab apparatus; Cryogenic Equipment – Cryogenic dewars, dry ...
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mitegen.com&d=DwIF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=A-U8hHexbpineZj5CPCin1wZ7eI3pGwhke9dRx44eCw&s=CDnEZPdF7jRmNBRLzwf3oq4YhKNVV5d5391OnxsN5iE&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mitegen.com&d=DwQF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=UnXiI86jUyBaYaIJaWvWNn_J7A27whVlq5swN45pW4w&e=>


best regards,

Richard

Richard M. Walsh , Ph.D.

The Harvard Cryo-EM
Center for Structural Biology

Harvard Medical School
250 Longwood Avenue
SGM 102
Boston MA 02115

Phone: (617) 432-9042
Email:Richard_Walsh at hms.harvard.edu<mailto:Richard_Walsh at hms.harvard.edu>
Web: cryoem.hms.harvard.edu<https://urldefense.proofpoint.com/v2/url?u=http-3A__cryoem.hms.harvard.edu_&d=DwMF-g&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=VUuBFUPEngsryfz8lFsupolCcUBe4N3onXQrA9kYDuU&s=Rj45yjtnLOrsylhyd8CXkFT0xrNiYEKRWSKBY6zPtnM&e=>
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Grassucci, Robert <rg2502 at cumc.columbia.edu>
Sent: Friday, September 24, 2021 9:34 AM
To: 3DEM List <3dem at ncmir.ucsd.edu>
Subject: [3dem] Problem getting top off pin type autogrid grid boxes

Hi all
We have been having a problem where users tighten the tops of the autogrid grid boxes and the tool’s metal tip unscrews before the top does.  Has anyone found some way to better secure the metal tip of the grid box top tool so it won’t spin or ensured the grid box top would loosen before the tip of the tool?  Thank you.
Bob

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