[3dem] Uranyl acetate alternative

Thu Oct 21 13:30:12 PDT 2021

If the problem is acquiring uranyl acetate solutions but not its other
salts, then uranyl formate would be our suggestion. It has finer granule
than the acetate salt but stable only couple of days.

On Thu, Oct 21, 2021, 8:44 AM Lesley Calder <Lesley.Calder at crick.ac.uk>
wrote:

> I don't have too much experience with detergents, but when I have tried
> I've had most success with UA, so no escaping those pesky uranyl salts
> completely.
>
>
> ------------------------------
> *From:* Craig Yoshioka <yoshiokc at ohsu.edu>
> *Sent:* 21 October 2021 16:16
> *To:* Lesley Calder <Lesley.Calder at crick.ac.uk>
> *Cc:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* Re: [3dem] Uranyl acetate alternative
>
>
> *External Sender:* Use caution.
>
> This is great, thanks!  Out of curiosity do you have a goto alternative if
> looking at samples with detergents?
>
> On Oct 21, 2021, at 5:58 AM, Lesley Calder <Lesley.Calder at crick.ac.uk>
> wrote:
>
> It seems like quite a few of you are interested in the SST preparation
> protocol, so here it is attached.
>
> Lesley
> ------------------------------
> *From:* Metskas, Lauren Ann <lmetskas at purdue.edu>
> *Sent:* 21 October 2021 12:12
> *To:* Lesley Calder <Lesley.Calder at crick.ac.uk>
> *Subject:* Re: [3dem] [EXTERNAL] Uranyl acetate alternative
>
>
> *External Sender:* Use caution.
>
> Hello,
>
> I’d love your recipe for the conversion to the salt form if you’re willing
> to share it!  I’m just starting up a new lab at Purdue University, and have
> been thinking about switching to a non-radioactive stain.
>
> Thank you!  Best,
> Lauren Ann
>
>
> Lauren Ann Metskas, Ph.D.
>
> Assistant Professor
> Department of Biological Sciences
> Joint Appointment in Department of Chemistry
> Purdue University
> Hockmeyer Hall (HOCK) Room 231
> 240 S Martin Jischke Dr.
> West Lafayette, IN 47907
>
> (Office) (765) 49-44909
> metskas at purdue.edu
>
>
>
> On Oct 21, 2021, at 5:15 AM, Lesley Calder <Lesley.Calder at crick.ac.uk>
> wrote:
>
> In 35 years of negative staining my go to stain is sodium silicotungstate.
> It provides good contrast has a fine grain, and therefore reveals details
> not obvious with uranyl salts and is great for really small molecules, it
> has none of the problems of radioactivity for handling and disposal. I use
> it with all the conventional buffers including PBS, but it's of no use when
> detergent is present.
> UK suppliers sell the acid form (not sure about anywhere else), and to
> achieve the best affects you need to neutralise your solution. I prefer to
> prepare a batch of the salt from the acid solution and precipitate it out
> to keep the solid for later use ... it keeps for years that way. I then
> prepare a 1% solution of the salt form, which also keeps for many months.
> If anyone would like the recipe for the conversion to the salt form, I'd
> be happy to supply it.
>
> Lesley Calder
>
> The Francis Crick Institute
> 1 Midland Road
> London NW1 1AT
>
>
>
> ------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Riedel, Dietmar <
> driedel at gwdg.de>
> *Sent:* 20 October 2021 14:32
> *To:* Sharon G Wolf <Sharon.Wolf at weizmann.ac.il>
> *Cc:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* Re: [3dem] [EXTERNAL] Uranyl acetate alternative
>
>
> *External Sender:* Use caution.
>
> I use NanoVan or NanoW. NanoVan is great for negativ stain if phosphates
> omit the use of Uran stains. But it is a vanadium stain and therefore much
> weaker than Uran salt. I keep it in the fridge for years.
> Dietmar
>
> Mobil gesendet
>
> Am 20.10.2021 um 15:07 schrieb Sharon G Wolf <Sharon.Wolf at weizmann.ac.il>:
>
> 
> I have used sodium silicotungstate on a variety of samples.  Also
> phosphotungstic acid is a classic.
> Also, like Zuben, I have used ammonium molybdate and methylamine
> tungstate.   Both need to be made fresh. They can’t sit on the shelf for a
> long time like UAc can.
> None of them has as much contrast as UAc, except for the sodium
> silicotungstate, which can be outstanding. But it can cause some complexes
> to fall apart.
> Sharon
>
>
>
>
>
>
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> *On Behalf Of *Brown, Zuben
> *Sent:* Wednesday, 20 October, 2021 15:41
> *To:* Denis Chrétien <denis.chretien at univ-rennes1.fr>; 3dem at ncmir.ucsd.edu
> *Subject:* Re: [3dem] [EXTERNAL] Uranyl acetate alternative
>
>
> Hi Denis,
>
>
> I've tried ammonium molybdate and methylamine tungstate with acceptable
> results on different membrane proteins.
> Obviously, the contrast is reduced compared to uranyl acetate but the
> management of the waste materials is greatly simplified.
>
>
> best wishes,
> Zuben
> ------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Denis Chrétien <
> denis.chretien at univ-rennes1.fr>
> *Sent:* Wednesday, October 20, 2021 3:35 AM
> *To:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* [EXTERNAL] [3dem] Uranyl acetate alternative
>
>
> Dear all,
>
>
> It becomes difficult to posses Uranyl Acetate salts. Hence we would like
> to have your feedback concerning alternatives for sections and negative
> staining. Along this line, we would appreciate your experience concerning
> this salt :
>
>
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=SPdUOA17Xfzp8a9up7qz99EKUtEaFKB47zvk_4_c-Y0&s=JEspDn9NHQh7gotUQY6EVqQ221VDENqdMHXqOTfGCqc&e= 
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>
>
> Other suggestions are welcomed!
>
>
> Best,
>
>
> Denis
>
> ___________________________
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