[3dem] [EXTERNAL] Uranyl acetate alternative

[Brown, Zuben]

Hi Denis,

I've tried ammonium molybdate and methylamine tungstate with acceptable results on different membrane proteins.
Obviously, the contrast is reduced compared to uranyl acetate but the management of the waste materials is greatly simplified.

best wishes,
Zuben
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Denis Chrétien <denis.chretien at univ-rennes1.fr>
Sent: Wednesday, October 20, 2021 3:35 AM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [EXTERNAL] [3dem] Uranyl acetate alternative

Dear all,

It becomes difficult to posses Uranyl Acetate salts. Hence we would like to have your feedback concerning alternatives for sections and negative staining. Along this line, we would appreciate your experience concerning this salt :

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwIFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=aOZVpo4udQ3YEHEMvedTwEtuCDBoLZKZ_I6ZgGCtsYY&s=vR1vNID5aU8yePh535dYWOX3qvRFZB4py7BLLDVzcRk&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=SzdIxK_0wo-0i8bTfRNyPQnZX2l3AyLDYJwGNuZsMTs&e=>

Other suggestions are welcomed!

Best,

Denis
___________________________
Université de Rennes 1
UMR 6290, équipe TIPs
Campus de Beaulieu, Bât. 13
35042 Rennes Cedex
France

Tel. +33 (0)2 23 23 67 64
https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwIFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=aOZVpo4udQ3YEHEMvedTwEtuCDBoLZKZ_I6ZgGCtsYY&s=pw6_qAXlXKDduCrIv0nCZRTyEAPvF2eelPqllIXIURM&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=J9NRrJh9gl0QKmDtkcBNTdu6xKGp5rtgq6N8Gs-jWos&e=>
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