[3dem] 3dem Digest, Vol 162, Issue 3

[Cazey, Cornelia]

Hi Dan,

There is some movement in the tool, but it generally helps already to simply tighten the screw tip into the holder shaft. If you have to use so much force to open/close the clipring that you unscrew the tip from the handle, you may be looking at a different problem alltogether. Generally, the clipring should easily be "caught" by the screw tip and "unclipped", there should not be further screwing motion after the tool and the clipring have engaged. When clipping the ring back in (motion in reverse), you should be able to unscrew the tool without any force, after the ring is seated correctly. The lighter the touch, the better, once the clipring has engaged, you should not need to apply further downward force at all and the tool should unscrew easily. Does that help?

Good luck and best wishes,

Cornelia

Centre for Structural Systems Biology (CSSB) 
c/o DESY, Building 15 
Notkestr. 85 
22607 Hamburg 
Germany 
phone: +49-(0)40-8998-87706 
email: cornelia.cazey at cssb-hamburg.de 

"If you don't know it's impossible, it's easier to do" -Neil Gaiman

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Subject: 3dem Digest, Vol 162, Issue 3

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Today's Topics:

   1. Gatan cryo tool screw loose (Dan Richman)


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Message: 1
Date: Mon, 1 Feb 2021 22:40:38 +0000
From: Dan Richman <d.e.richman at jhu.edu>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Gatan cryo tool screw loose
Message-ID:
	<MN2PR01MB6016B03BDC1CEF0B83C1D01EA4B69 at MN2PR01MB6016.prod.exchangelabs.com>
	
Content-Type: text/plain; charset="iso-8859-1"

Hi 3DEMers,
I'm trying to use the Gatan cryo tool, screw-tip version (not the wings that pivot), and I'm finding that the screw tip itself rotates and thus retracts into the plastic tool body while trying to engage the screw into the STP Clipring threads. This makes it difficult to manipulate the clipring and easy for clipring to fall off. Is this a feature or a bug? Has anyone taken the drastic step of gluing the screw tip into a fixed position?

Thanks,
Dan

--
Dan Richman, PhD   (he/him/his)
Postdoc Research Fellow, Berger Lab
Dept of Biophysics, Johns Hopkins School of Medicine
mobile: 201-669-0967
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