[3dem] strange ice pattern in grid

[Sharon G Wolf]

Hello
Indeed as Henning stated, its “Leopard ice”.
Are you using a side entry holder? It could be the clip ring holding down the grid is not tight, or the inner spring needs replacing. We also had issues with our Gatan 914 holder with Leopard ice that was only solved by having the holder serviced and the tip replaced. If it is an autoloader system, indeed it could be faulty clipping problems.
Good luck,
Sharon

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of ABBY
Sent: Saturday, 18 December, 2021 16:22
To: Stahlberg Henning <henning.stahlberg at epfl.ch>
Cc: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] strange ice pattern in grid

Hi Henning,

Thanks for your information. But even in the same hole, some area contain the wave shape ice and some area don't. While collecting data the temperature in TEM user interface is all OK so I am not sure the ice is affeted by EM temperature.
I use Quantifoil R1.2/1.3 Cu 300 mesh from the same batch. Maybe try other grid type is a good idea.

Best,
Yutong Song



At 2021-12-18 21:53:55, "Stahlberg Henning" <henning.stahlberg at epfl.ch<mailto:henning.stahlberg at epfl.ch>> wrote:
Hi,
I’d think your wave is Leopard skin contamination, where you sample in the EM is not cold enough. Water sublimates and recrystallizes nearby.
The other type could be residues from the grids. Did you try changing to another grid type?

Henning


On 18 Dec 2021, at 14:31, ABBY <aaabey at 126.com<mailto:aaabey at 126.com>> wrote:

Hi everyone,

We have seen some strange ice in our micrographs from K3 like attachments. One is grain shape ice and another is wave shape ice, some area contain them both. We tried to get tilt series and made tomograms, finding the strange pattern only exist in the bottom but not go through ice layer. Have anyone seen this before and know how these strange ice form? I think there is no problem in our grids recycle and storage.

Thanks a lot!

Best regards,
Yutong Song





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