[3dem] strange ice pattern in grid

[ABBY]

Hi everyone,


We have seen some strange ice in our micrographs from K3 like attachments. One is grain shape ice and another is wave shape ice, some area contain them both. We tried to get tilt series and made tomograms, finding the strange pattern only exist in the bottom but not go through ice layer. Have anyone seen this before and know how these strange ice form? I think there is no problem in our grids recycle and storage.


Thanks a lot!


Best regards,
Yutong Song


 
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20211218/dda6409a/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: micrographs.png
Type: image/png
Size: 1913254 bytes
Desc: not available
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20211218/dda6409a/attachment-0002.png>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: tomo_slice.png
Type: image/png
Size: 644980 bytes
Desc: not available
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20211218/dda6409a/attachment-0003.png>