[3dem] Relion issue Autopick - 2D classification

[Sjors Scheres - MRC LMB]

Hi Lucia,

Have you gone through the RELION tutorial? (see
https://urldefense.com/v3/__https://www3.mrc-lmb.cam.ac.uk/relion__;!!Mih3wA!SjMZhUKEds9bXVNVg1JqtNL0L0_PTG5s5qZ0Sy5m35KqVrPIb8vR1FWoyGizN8GBYg$ )

That's usually a good place to start learning RELION.

HTH,

Sjors


On 4/22/21 11:17 AM, Lucia wrote:
> Hi all,
>
> Sorry for bother and thanks for your patience/help. I’m trying to use
> Relion to Autopick (Laplacian) a set of elongated particles that are
> around 150A long. I managed to get a nice set of 2D classes in
> Cryosparc but, since I have preferential orientation, people recommend
> me to use Relion to redo my dataset analysis.
>
> But... I'm really stuck, I have no experience with Relion and I don't
> manage to get any good autopicking that lead me to a 2D classification
> (even a preliminar one to re do the picking after), I just get black
> images or nonsense in the 2D classification, even when I check the
> images after the autopicking and they don't look bad.
>
> Honestly idk what to put here about the parameters that I'm using
> because I tried a bunch but blindly (since I have no idea neither
> experience).
>
> I was hoping someone could give me some suggestions that help me to
> overcome that point because I'm desperate.
>
> Thanks!
> Lucia
>
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-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
https://urldefense.com/v3/__http://www2.mrc-lmb.cam.ac.uk/groups/scheres__;!!Mih3wA!SjMZhUKEds9bXVNVg1JqtNL0L0_PTG5s5qZ0Sy5m35KqVrPIb8vR1FWoyGgUzs4MRQ$