[3dem] 3dem Digest, Vol 164, Issue 19
Jason Kaelber
jason.kaelber at rutgers.edu
Tue Apr 13 13:45:13 PDT 2021
Regarding the beam drift issues--when you say "Magnetic Field compensation is always on," do you use both AC and DC field compensation? It is common for installs that only need AC comp to not have a DC sensor. If something changed later, (e.g. replacement of an elevator motor, installation of a breaker rack with large load fluctuations) it could introduce a changing DC field.
Also, how are your lens temperatures?
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Today's Topics:
1. Re: Talos Arctica 200 kV Beam Drift Issues (Carolin Seuring)
2. Postdoc position tomography McKee lab Montreal
(Marc D McKee, Professor)
----------------------------------------------------------------------
Message: 1
Date: Tue, 13 Apr 2021 20:23:35 +0200
From: Carolin Seuring <carolin.seuring at cssb-hamburg.de>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Talos Arctica 200 kV Beam Drift Issues
Message-ID: <1FD06905-D313-4DCB-AC06-8E295B67C00D at cssb-hamburg.de>
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Thanks Henning for the thoughts, and everyone with a similar issue:
> On 13. Apr 2021, at 16:22, Stahlberg Henning <henning.stahlberg at epfl.ch> wrote:
>
> Dear Carolin,
>
> You describe the drift of the beam in tens of nanometers. Is that in total, or per second or per exposure?
The beam is leaving the FOV within minutes, meaning we can see the fringes coming into the image within minutes.
The drift is in the order of 50 nm/min sometimes, but that varies.
> And you are speaking about beam drift, not image drift, right?
The image position/stage is stable, the beam is moving.
> If you simply widen the beam so that it is a micrometer in diameter, then 10nm drift should not matter....? Or why is it making life complicated?
True, when the beam is condensed it is worse, and in our case we are working with 50 or 70 um C2 apertures and they are giving us beams of either 1.8 or 2.6 um.
>
> If it is beam shift, how do you detect that? Is that from the flucam of the Arctica or from a DED at the bottom?
Yes both. Plus we can correct with MF-BS.
> Just to exclude that you have a Moir? effect between a 50Hz disturbance from AC fields and a close camera readout frequency: Does the beam shift look the same if you use different flucam readout speeds?
>
> If it is beam shift, what about beam tilt? And where does that shift, up in the gun or only closer to the sample at the level of the beam deflectors above the sample?
>
> Sometimes it is easier not to try to make matters better but worse, and that way you at least know where the problem comes from. Any parameter that is causing trouble for you will be at its limiting edge of the tolerance range. If you disturb that more to the slightest amount, you should immediately see the impact. If you instead disturb a parameter that is in a safe zone, then your drift problem should not be affected too much from that. So, you could touch with a warm hand all kinds of water lines or cables or vacuum tubes or warm parts of the column that are exposed to air flow, etc., to find the one parameter that is directly linked to the drift problem.
> Other than that, you could look into correlation with pressure changes in air or water, or temperature changes in these, or vacuum changes, or checking for a bending FEG tip.
Thanks for the great details - these are all really good ideas to explore!
Also for others in the community to complete the list of possible params: I got another really good input from someone who had an issue with the objective lens was physically loose in the microscope and charging at the start of data collection was causing it to move, but as the collection proceeded it reached the edge of the range of movement and then stabilised. This also sounds very familiar to our situation. It seems a rare issue though.
Thanks everyone who replied! This is so good!
Cheers,
Carolin
>
> Best,
> Henning.
>
>
> Henning Stahlberg
> Prof., Faculty of Biology and Medicine, UNIL, and
> Prof. for Physics, LBEM, IPHYS, SB, EPFL
> CH-1015 Lausanne, Switzerland
> mailto:henning.stahlberg at epfl.ch
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>
>
> ?On 13.04.21, 16:06, "3dem on behalf of Carolin Seuring" <3dem-bounces at ncmir.ucsd.edu on behalf of carolin.seuring at cssb-hamburg.de> wrote:
>
> Hi 3DEM community,
>
> We are looking for support in tracking down a beam drift issue on our Arctica Talos 200kV FEG system.
>
> The problem: For the better part of the past 6 months, and mostly during the day, the beam drifts over a few hours in one direction, and then randomly backwards - never getting back to its original position (but close). The drift is in the tenth of nanometers and makes data acquisition impossible during that time. The beam is stabilising often in the late evenings. Magnetic Field compensation is always on. The facility also houses 2 Krioses in separate rooms adjacent and further away from the Arctica room, but zero issues there.
>
> Parameters we have checked so far:
> - room temperature stability -> humidity controlled environment with airconditioning, 21-21.5?C
> - operator in the room vs operator on external remote controls -> using remote access from a separate room seems to cause less issues
> - air movement in the room -> seems negligible, only slow air movements towards air exchange vents
> - dewar filling time -> beam seems to drift less after dewar filling
> - whether centrifuges and instrumentation one floor up above the microscope are on ?> results not conclusive
> - tested efficiency of magnetic field compensation a two points in the room ?> ok
> - we used a carton paper to cover the 2m^2 Arctica top (thanks to Wim, who posted that somewhere)
> - we changed the C2 aperture set, that seemed to helped a bit a few months back
>
> Beam drift is now still on about once a day at fairly random times! So annoying?
>
> Has anyone encountered anything like this or has any other suggestions or parameters we should consider?
>
> Thanks so much for your help!
> Carolin
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------------------------------
Message: 2
Date: Tue, 13 Apr 2021 18:30:40 +0000
From: "Marc D McKee, Professor" <marc.mckee at mcgill.ca>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Postdoc position tomography McKee lab Montreal
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The research group of Marc D. McKee at McGill University in Montreal is seeking a postdoctoral fellow to perform structural biology studies on bones and teeth using a workflow of X-ray (Zeiss Xradia Versa 520), FIB-SEM (FEI Helios Nanolab 660 DualBeam) and APT (Cameca Leap 5000XR with cryo-stage) tomography, and Dragonfly 3D reconstruction software. An emphasis will be given to correlative cryo-workflow in this methodological pathway. This structural biology program uses predominantly normal and transgenic mutant mouse samples that are models of human mineralization diseases (human biopsy and surgical waste samples are also being similarly analyzed in a correlative manner).
For group leaders, as you deem appropriate, please forward this to any of your postdocs or senior PhD students that may be interested in this opportunity as part of their research interests and career trajectory.
I can be contacted at:
marc.mckee at mcgill.ca<mailto:marc.mckee at mcgill.ca>
And my university website is:
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Thank you ... Marc.
Marc D. McKee, Ph.D. | Professor
Canada Research Chair in Biomineralization<https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.chairs-chaires.gc.ca*2Fchairholders-titulaires*2Fprofile-eng.aspx*3FprofileId*3D4594__*3B!!Mih3wA!R5DOoz6_dAphjEUMdUO9IaLRCz8JbwFBZE4vHsSbY_VjrZHVOMNfa9l71zQ_8gHa2Q*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Ca5ef3df151004c9192e908d8feae5f41*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C1*7C637539372160166144*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C2000&sdata=zOYqor*2F*2FGXx4W6TtP*2BjBcnsSiHNRkAsMW*2Fy46tZiqos*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!WDeiu6e6mvMPX_YULJqk2v1MljjtASfBy-dFtX3_bYei4ltbRo1UHpLYxhcFDkTzNQ$ >
Faculty of Dentistry, and Faculty of Medicine and Health Sciences
Department of Anatomy and Cell Biology, School of Biomedical Sciences
McGill University, Montreal, Quebec, Canada
Contact Info: Mail and courier address | Marc McKee, McGill University,
Strathcona Bldg Room M73, 3640 University Street, Montreal, QC, Canada H3A 0C7
Tel: 514-398-4400 ext. 00041 marc.mckee at mcgill.ca<mailto:marc.mckee at mcgill.ca>
McKee McGill Website<https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.mcgill.ca*2Fdentistry*2Fmarc-d-mckee__*3B!!Mih3wA!R5DOoz6_dAphjEUMdUO9IaLRCz8JbwFBZE4vHsSbY_VjrZHVOMNfa9l71zRNTFgcrA*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Ca5ef3df151004c9192e908d8feae5f41*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C1*7C637539372160166144*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C2000&sdata=IeqQxoOAuip27SVSfYUkT7hwmiGVaPVkHIg7GCJQr*2FQ*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!WDeiu6e6mvMPX_YULJqk2v1MljjtASfBy-dFtX3_bYei4ltbRo1UHpLYxhe4-Cwx-w$ > McKee Google Scholar Website<https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2F*2Fscholar.google.ca*2Fcitations*3Fuser*3D2DWhZWsAAAAJ*26hl*3Den__*3B!!Mih3wA!R5DOoz6_dAphjEUMdUO9IaLRCz8JbwFBZE4vHsSbY_VjrZHVOMNfa9l71zTTU2YXgA*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Ca5ef3df151004c9192e908d8feae5f41*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C1*7C637539372160176147*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C2000&sdata=2DhgsdZ5pRcQCZmRHpMb3rBIn708kJh0e1a1NRXjN8U*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!WDeiu6e6mvMPX_YULJqk2v1MljjtASfBy-dFtX3_bYei4ltbRo1UHpLYxhemWIDqzw$ > Map to Office & Lab<https://urldefense.com/v3/__https://nam02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2F*2Fmaps.google.com*2Fmaps*3Fq*3D3640*Rue*University*2C*Montreal*2C*QC*26hl*3Den__*3BKysrKw!!Mih3wA!R5DOoz6_dAphjEUMdUO9IaLRCz8JbwFBZE4vHsSbY_VjrZHVOMNfa9l71zTQ874Tug*24&data=04*7C01*7Cjason.kaelber*40rutgers.edu*7Ca5ef3df151004c9192e908d8feae5f41*7Cb92d2b234d35447093ff69aca6632ffe*7C1*7C1*7C637539372160176147*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C2000&sdata=BJHUHbubCRokLnnVgv1U*2Bh1DkIRVhdwuXiBUwgzDZgo*3D&reserved=0__;JSUlJSUlJSUlJSUqKiUqJSolJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!WDeiu6e6mvMPX_YULJqk2v1MljjtASfBy-dFtX3_bYei4ltbRo1UHpLYxhcBUNB-Xg$ >
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