[3dem] contamination from UltrAuFoil?

[Mathew Peet]

Dear Winnie,

These can be removed with 2% HCl. The method I've used is pick up each 
grid with tweezer and then dunk in 2% HCl, 0.2% HCl, millipore, acetone 
then IPA. Several seconds in each and enter and exit small container (~ 
25 ml) at different position. Dry by wicking tweezers and grid with 
filter paper in laminar flow hood and place on filter paper.

For immediate use in water I avoided the drying with solvents and this 
also worked.

Best regards,

Mathew Peet



On 12/10/2020 10:08, Winnie Ling wrote:
> Dear colleagues,
>
> The protein we work with behaves very well with UltrAuFoil but we are 
> getting some particles of around 1-2nm all over the grids, some grids 
> with more, some grids with less. We did not see the same particles 
> with classic quantifoils (but the protein aggregates around the edges 
> of the holes). Could the particles be Au debris from the UltrAuFoil?
>
> Have any of you had the same problem? Any solution?
>
> Thanks a lot,
>
> Winnie Ling
>
>