[3dem] question in post-processing

[Basil Greber]

Hi Mohamed,

Have you done CTF refinement, beam tilt in particular?

Basil



> Am 03.05.2020 um 07:37 schrieb Mohamed A Sayed <mohamed.sobhy at kaust.edu.sa>:
> 
> Hello,
> 
> I hope all is safe. If you would please advise on the following:
>  
> I made auto-refinement for a structure using auto-sampling settings: Initial angular sampling 3.7, local searching from auto-sampling 1.8. The output after convergence was "Final resolution (without masking) is: 3.26154)". When I generate a mask using binarisation threshold from 0.001 to 0.005, where 0.005 gave warning, the FSC curve has a prominent peak in the FSC plot. I tried changing these parameters and tried the output from other refinement job with bigger box but all give this peak.
> 
> my questions
> 1- What caused and how to get rid of this peak?
> 2- If this peak problem could not be fixed, can I use proceed with such post-process.star file for ctf refinement and polishing?
> 
> Thank you very much for your help and time. 
> 
> <pastedImage.png>
> 
> Last iteration before conversion: 
> 
>  CurrentResolution= 3.93402 Angstroms, which requires orientationSampling of at least 2.04545 degrees for a particle of diameter 220 Angstroms
>  Oversampling= 0 NrHiddenVariableSamplingPoints= 1305
>  OrientationalSampling= 0.46875 NrOrientations= 145
>  TranslationalSampling= 0.4605 NrTranslations= 9
> =============================
>  Oversampling= 1 NrHiddenVariableSamplingPoints= 41760
>  OrientationalSampling= 0.234375 NrOrientations= 1160
>  TranslationalSampling= 0.23025 NrTranslations= 36
> Expectation iteration 25
> <pastedImage.png>
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