[3dem] ThermoF cassette loading station, problems with cracked nitrogen bath

[Alpay Burak Seven]

We got this email from Kliment Verba in this email list couple of months
ago. I hope it helps.
----

kliment.verba at ucsf.edu
Hi everyone,

we had a couple of the loading stations for the autoloader (Krios, Arctica)
crack and leak at the bottom. FEI told us that they were not repairable and
would cost 15k a piece. I tried to be hip and 3D print the parts first but
in my experience they would all crack and leak after a couple of cooling
cycles. So, I made a 3D model for CNC milling and these work as good as the
original parts. We used the company called FATHOM (https://studiofathom.com/)
here in Oakland and within about 10 days they can mill these things out for
about $400 a piece. We tried aluminum, teflon and delrin. It seems like the
original material is Delrin and is probably the best choice. Aluminum is
definitely not optimal as it takes a while to cool and frosts over quickly.
Teflon doesn't deform as much as Delrin but other than that seems similar.
Here is the link to the zip archive of the 3D model in three different file
formats .igs, .stp, .ipt. The first two you can give to any 3D milling
service and they will be able to make the part. The .ipt is the Autodesk
Inventor 3D file, so, you can open and play/modify it. Autodesk Inventor 3D
is free (as I think are all the Autodesk products) for students/.edu
addresses. I also include a pdf file with instructions on how to
disassemble the station.

Link to the archive:
https://drive.google.com/open?id=1Hsr6HpDHOd3mbroT7kja8jTkjseITBg-

Klim

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On Mon, Jan 20, 2020, 4:53 AM Laurinmäki, Pasi A <
pasi.laurinmaki at helsinki.fi> wrote:

> Dear community,
>
> The plastic bath of our Talos Arctica cassette loading station has
> developed serious cracks and thus leaks the LN2 through the insulation onto
> the table.
> As the unit has not been abused in any way, I assume that other people
> might have encountered similar problems.
>
> I would be interested in your solutions to the problem. We have used
> plastic bag between the insulation and the cracked plastic vessel to reduce
> the leak, but that doesn't seem to provide a long term (or even mid term)
> solution.
>
> Best regards,
> Pasi
>
> <<<<<<<<<<<<<<<<<<<>>>>>>>>>>>>>>>>>>
>
> Pasi Laurinmäki MSc BEng
>
> Laboratory Engineer & Cryo EM specialist
>
>
> Electron Microscopy Unit
>
> Institute of Biotechnology
>
> P.O.Box 56 (Viikinkaari 9)
>
> FI-00014 University of Helsinki, Finland
>
> Tel +358 50 4483 546
>
> <<<<<<<<<<<<<<<<<<<>>>>>>>>>>>>>>>>>>
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> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
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