[3dem] CryoEM Grid Boxes

[Devrim Acehan]

I like the X openings as well. If one arm of the X is wider to accommodate clipped grids and the other narrow as before to keep the bare grids, one type of box could be used for both and the tight fit will limit the grid movement to prevent ice damage during shipping. If narrow arm is always radial or tangential, people will get use to this complication.

My best,
Devrim

Weill Cornell Medicine
Cryo-EM Core Facility

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Ruben Diaz <pindusito at gmail.com>
Sent: Friday, April 24, 2020 3:09 PM
To: Michael Godfrin <mgodfrin at nanosoftmaterials.com>
Cc: 3DEM Mailing List <3dem at ncmir.ucsd.edu>
Subject: [EXTERNAL] Re: [3dem] CryoEM Grid Boxes

I really like the grid boxes with an X slot, so that I can put the grid on whichever orientation is more convenient for my hand. If anything, it would be nice to have them a little wider to accommodate autogrids. I prefer the numbering to be counter-clockwise, and the main reason for this is that when you have loosened the lid to insert the grids, moving it in the counter-clockwise direction does not tighten the screw, but rotating in a clockwise direction does  (it is super annoying to have a tight lid when you have a grid that needs to go into a slot). As for colors, I think white is nice, with a Philips/straight screw that is not super small, which can be opened with the posterior edge of some tweezers, cardboard, etc (as screwdrivers tend to walk away when most needed).

Ruben.

On Fri, Apr 24, 2020 at 10:00 AM Michael Godfrin <mgodfrin at nanosoftmaterials.com<mailto:mgodfrin at nanosoftmaterials.com>> wrote:
Hello Everyone,

We plan on offering CryoEM grid boxes in the coming months, both the "traditional" design for unclipped grids with the diamond shaped pockets, as well as the "newer" box style for autogrids.  We are finalizing designs and would like to incorporate any features that would be beneficial. There are a variety of opinions on the numbering convention for the grid boxes.  The "traditional" numbering convention seems to be clockwise, whereas newer autogrid boxes have the "reading" convention, though we've heard many people disregard that and refer to the positions in the traditional clockwise sense.

We would love to hear feedback from the community in an effort to reach a consensus and perhaps a standard on how to number the grid boxes. For anyone that responds to this survey<https://urldefense.com/v3/__https://docs.google.com/forms/d/e/1FAIpQLSdrdWhws85V-ld03o_4DhgtdRjyHKAm8XMggNO7xYJOAWMUwQ/viewform?usp=sf_link__;!!Mih3wA!Sc3pzHYpeLzdf01i3xWLUeiv4F000Go5-CW5_BG2A3FEqxi3oNEErCNeAvWUFL236Q$>, we will give a 20% discount on their first purchase of grid boxes once we begin selling them.  We will also share the results with everyone here once we have them.

Thank you and we hope that everyone is staying well,
Mike



Michael Godfrin, PhD
President

NanoSoft
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Coventry, RI 02816
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