[3dem] CryoEM Grid Boxes

Fri Apr 24 11:52:13 PDT 2020

If we are already discussing it openly there is actually no real reason why not make 6 instead of only 4 slots. We use 6 slots boxes and it works and is so much handier. 

Cristina

________________________________________________________________________

Dr. Cristina Paulino
Assistant Professor 
Head of the cryo-Electron Microscopy Unit
Department of Structural Biology and Membrane Enzymology

Groningen Biomolecular Sciences and Biotechnology Institute (GBB)
Faculty of Science and Engineering, University of Groningen
Nijenborgh 4, 9747 AG Groningen, The Netherlands
Room 5116-0141
Phone: +31 50 363 34 02 
Email: c.paulino at rug.nl 

ORCID: 0000-0001-7017-109X
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________________________________________________________________________

>> On 24. Apr 2020, at 20:42, Matt Swulius <mts286 at psu.edu> wrote:
> 
> I second Mike's suggestions.
> 
> In addition, color choice for the plastic should be considered in terms of how easily unclipped grids can be seen under LN. Maybe it's different for each person, but some colors simply make seeing the grid under LN quite difficult. It's not a deal breaker for me, but certainly convenient.
> 
> Cheers,
> Matt
> 
>> On Fri, Apr 24, 2020 at 2:18 PM Michael Strauss, Dr <mike.strauss at mcgill.ca> wrote:
>> Clockwise from the notch is the obvious way to do this, because numbers are largely illegible under liquid nitrogen, but the notch is always evident.
>> 
>> Also, I’d suggest one clever design that accommodates both clipped and unclipped grids.  Less for the user to think about, and less futzing about when clipping a host of grids.  They go back in the box the came out of, only now clipped.
>> 
>> good luck.
>> mike
>> 
>> --------
>> Mike Strauss
>> Dep’t of Anatomy and Cell Biology
>> McGill University
>> 3640 University St.
>> Strathcona Anatomy Bldg, Rm 144
>> Montreal, QC H3A 0C7
>> (514) 398-8084
>> mike.strauss at mcgill.ca
>> 
>> > On 24. Apr 2020, at 12:59, Michael Godfrin <mgodfrin at nanosoftmaterials.com> wrote:
>> > 
>> > Hello Everyone,
>> > 
>> > We plan on offering CryoEM grid boxes in the coming months, both the "traditional" design for unclipped grids with the diamond shaped pockets, as well as the "newer" box style for autogrids.  We are finalizing designs and would like to incorporate any features that would be beneficial. There are a variety of opinions on the numbering convention for the grid boxes.  The "traditional" numbering convention seems to be clockwise, whereas newer autogrid boxes have the "reading" convention, though we've heard many people disregard that and refer to the positions in the traditional clockwise sense.  
>> > 
>> > We would love to hear feedback from the community in an effort to reach a consensus and perhaps a standard on how to number the grid boxes. For anyone that responds to this survey, we will give a 20% discount on their first purchase of grid boxes once we begin selling them.  We will also share the results with everyone here once we have them.
>> > 
>> > Thank you and we hope that everyone is staying well,
>> > Mike
>> > 
>> > 
>> > 
>> > Michael Godfrin, PhD
>> > President
>> > 
>> > NanoSoft
>> > 1372 Main St.
>> > Coventry, RI 02816
>> > Cell: (401) 829-5527
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