[3dem] your opinion on these images from graphene grids

[Reinhard Rachel]

>>> 20.11.2019 at 14:54:
> Jacopo,
> 
> Assuming these are pictures of a frozen specimen, I would assume the spots 
> are artifacts of likely radiation damage, especially if they bear no 
> resemblance to your protein prepared by negative staining.  The spots also 
> vary in size.  Does that resemble your protein?  If so, then you have a very 
> heterogeneous sample.

I can understand the comments by David (Belnap)  ... 
and take the opportunity to make (more) people aware of Theresa's and Arne's pictures and remarks on sample homo-/heterogeneity and background,  in JSB 203 (Sept 2018) 242-246
Theresa Gewering, D. Januliene, et al .... "Know your detergent ...."
I was for long not aware of this Techn.Note, but only had similar experiences ... 
kind regards - Reinhard

-- 
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
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D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel at biologie.uni-regensburg.de
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